Krahe Thomas E, Filgueiras Cláudio C, da Silva Quaresma Renata, Schibuola Helen Gomes, Abreu-Villaça Yael, Manhães Alex C, Ribeiro-Carvalho Anderson
Departamento de Ciências Fisiológicas, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Av. Prof. Manoel de Abreu 444, 5 andar - Vila Isabel, Rio de Janeiro, RJ, 20550-170, Brazil.
Departamento de Ciências, Faculdade de Formação de Professores da Universidade do Estado do Rio de Janeiro, Rua Dr. Francisco Portela 1470 - Patronato, São Gonçalo, RJ, 24435-005, Brazil.
Neurosci Lett. 2017 Jun 9;651:102-108. doi: 10.1016/j.neulet.2017.04.050. Epub 2017 Apr 26.
Mixing alcohol with energy drinks has become increasingly popular among teenagers and young adults due to the prevailing view that the stimulant properties of energy drinks decrease the depressant effects of alcohol. Surprisingly, in spite of energy drinks being heavily marketed to and consumed by adolescents, there is scarcely available preclinical data on the neurobehavioral effects of energy drinks mixed with alcohol during adolescence. Thus, here we examine the effects of the combined exposure to alcohol and energy drink on adolescent mice using a variety of behavioral tasks to assess locomotor activity, righting reflex and motor coordination. At postnatal day 40, male and female Swiss mice were assigned to the following experimental groups: alcohol diluted in energy drink (Ed+Etoh), alcohol diluted in water (Etoh) or controls (Ctrl: energy drink or water). Alcohol and energy drink (Red Bull) concentrations were 4g/kg and 8ml/kg, respectively, and all solutions were administered via oral gavage. When compared to Etoh mice, Ed+Etoh animals displayed greater locomotor activity and increased anxiety-like behaviors in the open-field, lost their righting reflexes sooner and displayed poorer motor coordination in the rotarod. Collectively, our findings indicate that alcohol-induced deficits in adolescent mice are worsened by energy drink and go against the view that the stimulant properties of energy drinks can antagonize the adverse effects of alcohol.
将酒精与能量饮料混合在青少年和年轻人中越来越流行，因为普遍认为能量饮料的刺激特性会降低酒精的抑制作用。令人惊讶的是，尽管能量饮料大量向青少年推销且被他们消费，但几乎没有关于青春期时能量饮料与酒精混合后的神经行为效应的临床前数据。因此，在这里我们使用各种行为任务来评估运动活动、翻正反射和运动协调性，研究酒精和能量饮料联合暴露对青春期小鼠的影响。在出生后第40天，将雄性和雌性瑞士小鼠分配到以下实验组：能量饮料中稀释的酒精（Ed+Etoh）、水中稀释的酒精（Etoh）或对照组（Ctrl：能量饮料或水）。酒精和能量饮料（红牛）的浓度分别为4g/kg和8ml/kg，所有溶液均通过灌胃给药。与Etoh小鼠相比，Ed+Etoh组动物在旷场试验中表现出更大的运动活动和增加的焦虑样行为，更快失去翻正反射，并且在转棒试验中表现出更差的运动协调性。总的来说，我们的研究结果表明，能量饮料会使青春期小鼠中酒精诱导的缺陷恶化，这与能量饮料的刺激特性可以对抗酒精的不利影响这一观点相悖。
