Asorey Lucas G, Carbone Silvia, Gonzalez Bárbara J, Cutrera Rodolfo A
Universidad de Buenos Aires, CONICET, Instituto de Fisiología y Biofísica "Bernardo Houssay" (IFIBIO), Buenos Aires, Argentina.
Universidad de Buenos Aires, CONICET, Departamento de Ciencias Fisiológicas, Buenos Aires, Argentina.
Neurosci Lett. 2018 Mar 23;670:1-7. doi: 10.1016/j.neulet.2018.01.030. Epub 2018 Feb 2.
In last few years it has been a significant increase in the consumption of alcohol combined with energy drink. The aim of this work was to study the effect of this mixture in motor and affective behaviors during an alcohol hangover episode. Male Swiss mice received one of the following treatments: saline + sucrose; saline + energy drink; ethanol + sucrose; ethanol + energy drink. Ethanol dose was 3.8 g/kg BW (i.p.) and energy drink dose was 18 ml/kg BW (gavage) at ZT1 (8 am) (ZT: Zeitgeber time; ZT0: 7 am; lights on). The behavioral tests used were tight rope test to determine motor coordination; hanging wire test to study muscular strength; elevated plus maze and open field tests to evaluate anxiety like-behavior and locomotor activity. Tests were carried out at basal point that matched with lights onset and every 6 h up to 18 h after treatments. Hangover onset was established at ZT7 when blood alcohol concentration (BAC) was almost zero. Our results showed that the mixture of alcohol and energy drink altered significantly motor skills. Specifically, a significant decrease was observed in the performance of the animals in the tightrope and hanging wire tests in groups treated with the mixture of alcohol and energy drink. A significant impairment in the anxiety-like behavior was observed mainly at the beginning of alcohol hangover. These findings suggest that energy drink added to alcohol extends motor disabilities observed during an alcohol hangover episode in comparison with animals that received alcohol alone.
在过去几年中，酒精与能量饮料混合饮用的情况显著增加。本研究的目的是探讨这种混合物在酒精宿醉期间对运动和情感行为的影响。雄性瑞士小鼠接受以下处理之一：生理盐水 + 蔗糖；生理盐水 + 能量饮料；乙醇 + 蔗糖；乙醇 + 能量饮料。乙醇剂量为3.8 g/kg体重（腹腔注射），能量饮料剂量为18 ml/kg体重（灌胃），给药时间为ZT1（上午8点）（ZT：授时时间；ZT0：上午7点；开灯时间）。所使用的行为测试包括：紧绳试验以确定运动协调性；悬线试验以研究肌肉力量；高架十字迷宫试验和旷场试验以评估焦虑样行为和运动活动。测试在与开灯时间匹配的基础时间点进行，并在处理后每6小时进行一次，直至18小时。当血液酒精浓度（BAC）几乎为零时，确定在ZT7出现宿醉。我们的结果表明，酒精与能量饮料的混合物显著改变了运动技能。具体而言，在接受酒精与能量饮料混合物处理的组中，动物在紧绳试验和悬线试验中的表现显著下降。主要在酒精宿醉开始时观察到焦虑样行为有显著损害。这些发现表明，与仅接受酒精的动物相比，添加到酒精中的能量饮料会延长酒精宿醉期间观察到的运动障碍。
