SCF复合体在牛早期胚胎发育过程中对合子基因组激活的潜在作用

Potential Involvement of SCF-Complex in Zygotic Genome Activation During Early Bovine Embryo Development.

作者信息

Benesova Veronika, Kinterova Veronika, Kanka Jiri, Toralova Tereza

机构信息

Laboratory of Developmental Biology, Institute of Animal Physiology and Genetics, Academy of Science of Czech Republic, v.v.i., Libechov, Czech Republic.

Faculty of Science, Charles University in Prague, Prague, Czech Republic.

出版信息

Methods Mol Biol. 2017;1605:245-257. doi: 10.1007/978-1-4939-6988-3_17.

DOI:10.1007/978-1-4939-6988-3_17

PMID:28456970

Abstract

Proper timing of degradation of maternal protein reserves is important for early embryonic development. The major modification that triggers proteins to degradation is ubiquitination, mediated by ubiquitin-proteolytic system. We focus here on Skp 1-Cul 1-F-box complex (SCF-complex), E3 ubiquitin-ligase, a part of ubiquitin-proteolytic system, which transfer ubiquitin to the substrate protein. We describe in this chapter the methods for the characterization of the expression profile of mRNA and protein of invariant members of SCF-complex and for the definition of SCF-complex activity.

摘要

母体蛋白质储备的适时降解对早期胚胎发育至关重要。触发蛋白质降解的主要修饰是由泛素 - 蛋白水解系统介导的泛素化。我们在此聚焦于泛素 - 蛋白水解系统的一部分——Skp 1 - Cul 1 - F盒复合物（SCF复合物），即E3泛素连接酶，它将泛素转移到底物蛋白上。在本章中，我们描述了用于表征SCF复合物不变成员的mRNA和蛋白质表达谱以及定义SCF复合物活性的方法。