Department of Polymer and Color Engineering, Amirkabir University of Technology, 424, Hafez St., P.O. Box: 15875-4413, Tehran, Iran.
Department of Polymer and Color Engineering, Amirkabir University of Technology, 424, Hafez St., P.O. Box: 15875-4413, Tehran, Iran.
Carbohydr Polym. 2017 Jul 15;168:14-21. doi: 10.1016/j.carbpol.2017.03.031. Epub 2017 Mar 10.
Purpose of this article is developing novel, inexpensive curcumin loaded chitosan nanoparticles with targeting ability. Curcumin loaded folate-modified-chitosan-nanoparticles (NPs) have been synthesized and fabricated via self-assembling process. Chemical structures of modified chains, nanoparticle size in dry and wet state, zeta potential, morphology of NPs, physical state of curcumin in NPs, drug release profile and cytotoxicity of NPs were investigated by FTIR, FE-SEM, DLS and XRD, UV-vis spectrophotometer, and MTT assay against L929 and MCF7 cell lines, respectively. Results show nanoparticle size in dry state varied in range of 119-127nm and curcumin was loaded into nanoparticles with 96.47% efficacy. Drug release studies showed by decreasing pH of release medium from 7.4 to 5, release rate of curcumin from NPs increased, which shows pH responsive capacity of folate-modified chitosan nanoparticles. Cell viability studies confirmed that curcumin loaded NPs have good potential as a drug delivery system for breast cancer therapy.
本文的目的是开发具有靶向能力的新型、廉价的载姜黄素壳聚糖纳米粒子。通过自组装过程合成并制备了载叶酸修饰壳聚糖纳米粒子（NPs）的姜黄素。通过傅里叶变换红外光谱（FTIR）、场发射扫描电子显微镜（FE-SEM）、动态光散射（DLS）和 X 射线衍射（XRD）、紫外可见分光光度计和 MTT 测定法分别研究了修饰链的化学结构、干态和湿态纳米粒子的粒径、Zeta 电位、NPs 的形态、NPs 中姜黄素的物理状态、药物释放曲线和 NPs 的细胞毒性，以 L929 和 MCF7 细胞系为研究对象。结果表明，干态纳米粒子的粒径在 119-127nm 范围内，姜黄素的载药效率为 96.47%。药物释放研究表明，随着释放介质 pH 值从 7.4 降低到 5，姜黄素从 NPs 中的释放速率增加，这表明叶酸修饰壳聚糖纳米粒子具有 pH 响应能力。细胞活力研究证实，载姜黄素 NPs 具有作为乳腺癌治疗药物递送系统的良好潜力。
