College of Marine Life Sciences, Ocean University of China, NO.5 Yushan Road, Qingdao 266003, PR China.
College of Marine Life Sciences, Ocean University of China, NO.5 Yushan Road, Qingdao 266003, PR China.
Int J Biol Macromol. 2018 Jun;112:413-421. doi: 10.1016/j.ijbiomac.2018.02.002. Epub 2018 Feb 2.
Polyelectrolyte complex nanoparticles were fabricated via electrostatic complexation between Enteromorpha prolifera polysaccharide (EP) and chitosan (CS). The monodisperse EP/CS NPs with negatively charge were prepared at pH4.0 and mixing ratio of 3.0:1.0. Curcumin loaded nanoparticles (CUR-NPs) showed spherical morphology with negatively charge of -16.27±0.97mV and average diameter range of 230 to 330nm. The results of spectroscopic analysis, XRD and DSC confirmed that there were hydrogen-bonding interaction and hydrophobic interaction between curcumin and EP/CS NPs. The CUR-NPs improved storage, thermal and photo stability of curcumin and exhibited sustained release of curcumin in vitro. Moreover, the CUR-NPs showed higher cellular uptake than free CUR with incubation for 3h by CLSM visualization and fluorescence quantitative assay. Furthermore, MTT assay results demonstrated that the CUR-NPs possessed good anticancer activity against B16F10 cells. Therefore, the EP-based nanoparticles are promising candidates for carriers in controllable hydrophobic anti-tumor drug delivery.
聚电解质复合纳米粒子是通过浒苔多糖（EP）与壳聚糖（CS）之间的静电络合作用制备的。在 pH4.0 和 3.0:1.0 的混合比下制备带负电荷的单分散 EP/CS NPs。载姜黄素纳米粒子（CUR-NPs）呈球形，带负电荷为-16.27±0.97mV，平均直径范围为 230 至 330nm。光谱分析、XRD 和 DSC 的结果证实，姜黄素与 EP/CS NPs 之间存在氢键相互作用和疏水相互作用。CUR-NPs 提高了姜黄素的储存、热稳定性和光稳定性，并表现出体外姜黄素的持续释放。此外，通过 CLSM 可视化和荧光定量分析，CUR-NPs 在孵育 3h 后显示出比游离 CUR 更高的细胞摄取率。此外，MTT 测定结果表明，CUR-NPs 对 B16F10 细胞具有良好的抗癌活性。因此，基于 EP 的纳米粒子是可控疏水性抗肿瘤药物递送载体的有前途的候选物。
