Frasca Daniela, Diaz Alain, Romero Maria, D'Eramo Francesca, Blomberg Bonnie B
Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, FL 33101, USA.
Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, FL 33101, USA.
Cell Immunol. 2017 Nov;321:68-73. doi: 10.1016/j.cellimm.2017.04.007. Epub 2017 Apr 24.
In order to compare human and mouse B cell subset markers, we evaluated T-bet expression in human B cell subsets from individuals of different ages. We found T-bet expressed in unstimulated memory more than naïve B cells, and more in young individuals. TLR7 stimulation up-regulated T-bet in all B cell subsets from young and elderly individuals, and more in the elderly. By fold-increase the best effect was seen in subsets of the elderly and especially in those that undergo class switch (naïve and IgM). We also evaluated CD11c expression, as T-bet+CD11c+ B cells are expanded in healthy elderly individuals and also in patients with autoimmunity. Similar to T-bet, CD11c expression was higher in memory than in naïve B cells, but no differences were observed between young and elderly individuals. After TLR7 stimulation, CD11c increases in all B cell subsets (especially in naïve and IgM) from the elderly.
为了比较人类和小鼠B细胞亚群标志物，我们评估了不同年龄个体的人类B细胞亚群中T-bet的表达情况。我们发现，未受刺激的记忆B细胞中T-bet的表达高于初始B细胞，且在年轻个体中表达更多。TLR7刺激使年轻和老年个体所有B细胞亚群中的T-bet上调，且在老年个体中上调幅度更大。通过计算上调倍数，在老年个体的亚群中，尤其是那些经历类别转换的亚群（初始B细胞和IgM）中，观察到最佳效果。我们还评估了CD11c的表达，因为T-bet+CD11c+ B细胞在健康老年个体以及自身免疫患者中均有扩增。与T-bet相似，记忆B细胞中CD11c的表达高于初始B细胞，但在年轻和老年个体之间未观察到差异。TLR7刺激后，老年个体所有B细胞亚群（尤其是初始B细胞和IgM）中的CD11c均增加。
