叶黄素干预对瑞士白化小鼠γ辐射诱导的细胞损伤的姑息作用。

Palliative effects of lutein intervention in gamma-radiation-induced cellular damages in Swiss albino mice.

作者信息

Vasudeva Vidya, Tenkanidiyoor Yogish Somayaji, Radhakrishna Vishakh, Shivappa Pooja, Lakshman Srikanth Patil, Fernandes Ronald, Patali Krishna Ananthapura

机构信息

Research Scholar, Central Research Laboratory, K S Hegde Medical Academy, Nitte University, Mangalore, Karnataka, India.

Department of Physiology, K S Hegde Medical Academy, Nitte University, Mangalore, Karnataka, India.

出版信息

Indian J Pharmacol. 2017 Jan-Feb;49(1):26-33. doi: 10.4103/0253-7613.201013.

DOI:10.4103/0253-7613.201013

PMID:28458419

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5351234/

Abstract

OBJECTIVES

Radiation-induced hematological, biochemical, and cytogenetic damages to the normal cells are major concerns in the field of radiotherapy. The carotenoids and their derivatives have been the source of antioxidants with wide range of medicinal applications. The objective is to evaluate the protective effects of lutein, a carotenoid, against radiation-induced cellular and tissue damages.

METHODS

Swiss albino mice were grouped into 5, 50, 250, and 500 mg/kg b.wt. of lutein treatment groups, a sham and vehicle control group. The groups were irradiated with a lethal dose of 10 Gy y'-radiation. The mortality was recorded for 30 days to optimize the protective dose against radiation. The mice were administered with the compound orally for 15 consecutive days and irradiated with a sublethal dose of 6Gy. The hematological changes in blood and antioxidant parameters were determined in liver, kidney homogenates, and hemolysate/serum. The hematological parameters were recorded using an automated cell counter. The antioxidants such as malondialdehyde (MDA), glutathione (GSH), superoxide dismutase, catalase, glutathione peroxidase and glutathione-S-transferase were spectrophotometrically determined.

RESULTS

The red blood cell, white blood cell count, lymphocyte count, hemoglobin, platelet levels, and hematocrit value were found to be decreased in the irradiated groups. Lutein pretreatment maintains near-normal levels of these parameters indicating resistance/recovery from the radiation-induced damages. The antioxidant levels were found to be reduced in all the irradiated groups. However, lutein pretreatment (50 mg/kg b.wt.) has increased the catalase activity of hemolysate. Lutein pretreatment has reduced the MDA levels in hemolysate, when administered at doses of 5, 250, and 500 mg/kg b.wt. in comparison to its control.

CONCLUSION

The study demonstrates the radioprotective potential of lutein by maintaining the hematological and antioxidant homeostasis.

摘要

目的

放疗领域中，辐射对正常细胞造成的血液学、生化及细胞遗传学损伤是主要关注点。类胡萝卜素及其衍生物一直是具有广泛医学应用的抗氧化剂来源。本研究旨在评估类胡萝卜素叶黄素对辐射诱导的细胞和组织损伤的保护作用。

方法

将瑞士白化小鼠分为叶黄素处理组，剂量分别为5、50、250和500mg/kg体重，以及假照射组和溶剂对照组。所有组均接受10Gyγ射线的致死剂量照射。记录30天内的死亡率，以优化辐射防护剂量。连续15天给小鼠口服该化合物，然后给予6Gy的亚致死剂量照射。测定血液中的血液学变化以及肝脏、肾脏匀浆和溶血产物/血清中的抗氧化参数。使用自动血细胞计数器记录血液学参数。通过分光光度法测定抗氧化剂，如丙二醛（MDA）、谷胱甘肽（GSH）、超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽-S-转移酶。

结果

照射组的红细胞、白细胞计数、淋巴细胞计数、血红蛋白、血小板水平和血细胞比容值均下降。叶黄素预处理使这些参数维持在接近正常水平，表明对辐射诱导的损伤具有抗性/恢复能力。所有照射组的抗氧化剂水平均降低。然而，叶黄素预处理（50mg/kg体重）提高了溶血产物中的过氧化氢酶活性。与对照组相比，给予5、250和500mg/kg体重剂量的叶黄素预处理降低了溶血产物中的MDA水平。