Meyer Julie L, Paul Valerie J, Raymundo Laurie J, Teplitski Max
Soil and Water Science Department, University of Florida-Institute of Food and Agricultural SciencesGainesville, FL, USA.
Smithsonian Marine StationFort Pierce, FL, USA.
Front Microbiol. 2017 Apr 18;8:618. doi: 10.3389/fmicb.2017.00618. eCollection 2017.
Black Band Disease (BBD), the destructive microbial consortium dominated by the cyanobacterium , affects corals worldwide. While the taxonomic composition of BBD consortia has been well-characterized, substantially less is known about its functional repertoire. We sequenced the metagenomes of Caribbean and Pacific black band mats and cultured and obtained five metagenome-assembled genomes (MAGs) of , nine of Proteobacteria, and 12 of Bacteroidetes. Genomic content analysis suggests that is a source of organic carbon and nitrogen, as well as natural products that may influence interactions between microbes. Proteobacteria and Bacteroidetes members of the disease consortium are suited to the degradation of amino acids, proteins, and carbohydrates. The accumulation of sulfide underneath the black band mat, in part due to a lack of sulfur oxidizers, contributes to the lethality of the disease. The presence of sulfide:quinone oxidoreductase genes in all five MAGs and in the MAGs of several heterotrophs demonstrates that resistance to sulfide is an important characteristic for members of the BBD consortium.
黑带病（BBD）是一种以蓝细菌为主导的具有破坏性的微生物群落，影响着全球的珊瑚。虽然黑带病群落的分类组成已得到充分表征，但其功能库却知之甚少。我们对加勒比海和太平洋黑带菌席的宏基因组进行了测序，并培养获得了5个蓝细菌的宏基因组组装基因组（MAGs）、9个变形菌门的MAGs和12个拟杆菌门的MAGs。基因组内容分析表明，蓝细菌是有机碳、氮以及可能影响微生物间相互作用的天然产物的来源。疾病群落中的变形菌门和拟杆菌门成员适合降解氨基酸、蛋白质和碳水化合物。黑带菌席下方硫化物的积累，部分原因是缺乏硫氧化菌，这导致了该疾病的致命性。所有5个蓝细菌MAGs以及几个异养菌的MAGs中存在硫化物：醌氧化还原酶基因，这表明对硫化物的抗性是黑带病群落成员的一个重要特征。
