Browning G F, Ficorilli N, Studdert M J
School of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia.
Arch Virol. 1988;101(3-4):183-90. doi: 10.1007/BF01310999.
Two previously unknown and distinct herpesviruses were isolated from donkeys. One, with the characteristics of a betaherpesvirus, was isolated from the leukocytes of an apparently healthy donkey, while the second, an alphaherpesvirus, was recovered from the nasal cavity of donkeys given high doses of corticosteroids, and caused rhinitis in two seronegative weanling donkeys when they were intranasally infected. Few, if any, restriction endonuclease fragments were shared by the donkey betaherpesvirus, equine herpesvirus 2 (EHV 2) or EHV 5, a second distinctly different equine betaherpesvirus, nor by the donkey alphaherpesvirus, EHV 1, EHV 4, or EHV 3. In Southern blot analysis the donkey betaherpesvirus showed low levels of sequence similarity to both EHV 2 and EHV 5, while the donkey alphaherpesvirus and EHV 1 shared a moderate degree of sequence similarity, less similarity with EHV 4 and very low level of sequence similarity with EHV 3. These two isolates appear prototypic of two previously unrecorded herpesviruses for which the names asinine herpesvirus 2 and 3 are suggested for the betaherpesvirus and the alphaherpesvirus respectively.
从驴身上分离出两种此前未知且不同的疱疹病毒。一种具有β疱疹病毒的特征，是从一头看似健康的驴的白细胞中分离出来的；而另一种α疱疹病毒，则是从接受高剂量皮质类固醇的驴的鼻腔中分离出来的，当两只血清阴性的断奶幼驴经鼻内感染该病毒时，引发了鼻炎。驴β疱疹病毒、马疱疹病毒2（EHV 2）或EHV 5（另一种截然不同的马β疱疹病毒），以及驴α疱疹病毒、EHV 1、EHV 4或EHV 3，几乎没有（如果有的话）共同的限制性内切酶片段。在Southern印迹分析中，驴β疱疹病毒与EHV 2和EHV 5的序列相似性较低，而驴α疱疹病毒与EHV 1有一定程度的序列相似性，与EHV 4的相似性较低，与EHV 3的序列相似性极低。这两种分离株似乎是两种此前未记录的疱疹病毒的原型，建议分别将β疱疹病毒和α疱疹病毒命名为驴疱疹病毒2和3。
