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摩洛哥马疱疹病毒1型（EHV-1）和马疱疹病毒4型（EHV-4）的首次分子与系统发育特征分析

First Molecular and Phylogenetic Characterization of Equine Herpesvirus-1 (EHV-1) and Equine Herpesvirus-4 (EHV-4) in Morocco.

作者信息

El Brini Zineb, Cullinane Ann, Garvey Marie, Fassi Fihri Ouafaa, Fellahi Siham, Amraoui Farid, Loutfi Chafiqa, Sebbar Ghizlane, Paillot Romain, Piro Mohammed

机构信息

Department of Medicine, Surgery, and Reproduction, Agronomy and Veterinary Institute Hassan II, Rabat 10000, Morocco.

Virology Unit, The Irish Equine Centre, Johnstown, Naas, Co., W91 RH93 Kildare, Ireland.

出版信息

Animals (Basel). 2025 Jan 5;15(1):102. doi: 10.3390/ani15010102.

DOI:10.3390/ani15010102
PMID:39795045
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11718982/
Abstract

This study aimed to investigate the molecular prevalence and genetic characterization of EHV-1 and EHV-4 in equid populations in Morocco. A total of 154 equids (114 horses, 9 donkeys, and 31 mules) were sampled, with nasal swabs and tissue samples subjected to multiplex real-time PCR for the detection of EHV-1 and EHV-4. Additionally, an isolate from the tissue of an aborted horse fetus was included in the analysis. A subset of EHV-positive samples underwent virus isolation followed by whole-genome sequencing. PCR assays revealed that 42 samples (27%) tested positive for EHV-4, while only 3 samples (1.94%) were positive for EHV-1. Attempts to isolate the virus from EHV-4-positive samples were unsuccessful. However, virus isolation was successful in an EHV-1-positive nasopharyngeal sample from a donkey. Phylogenetic and molecular characterization reclassified the EHV-1 isolated from the donkey as an EHV-8. Meanwhile, the EHV-1 isolated from the aborted fetal tissue was classified as a clade 1 EHV-1 virus. This study is the first to report the molecular prevalence and genetic characterization of EHV-1 and EHV-4 in equid populations in Morocco, providing valuable insights into the distribution and genetic diversity of these viruses in the region.

摘要

本研究旨在调查摩洛哥马属动物群体中EHV - 1和EHV - 4的分子流行情况及基因特征。共采集了154份马属动物样本（114匹马、9头驴和31头骡），鼻拭子和组织样本采用多重实时PCR检测EHV - 1和EHV - 4。此外，分析中还纳入了一份流产马胎儿组织的分离株。对一部分EHV阳性样本进行病毒分离，随后进行全基因组测序。PCR检测显示，42份样本（27%）EHV - 4检测呈阳性，而只有3份样本（1.94%）EHV - 1呈阳性。从EHV - 4阳性样本中分离病毒的尝试未成功。然而，从一头驴的EHV - 1阳性鼻咽样本中成功分离出病毒。系统发育和分子特征分析将从驴身上分离出的EHV - 1重新分类为EHV - 8。同时，从流产胎儿组织中分离出的EHV - 1被归类为1型EHV - 1病毒。本研究首次报告了摩洛哥马属动物群体中EHV - 1和EHV - 4的分子流行情况及基因特征，为该地区这些病毒的分布和遗传多样性提供了有价值的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/11718982/6f39638dbee5/animals-15-00102-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/11718982/903244b20cc8/animals-15-00102-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/11718982/091c36195651/animals-15-00102-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/11718982/a291ad164b15/animals-15-00102-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/11718982/6f39638dbee5/animals-15-00102-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/11718982/903244b20cc8/animals-15-00102-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/11718982/091c36195651/animals-15-00102-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/11718982/a291ad164b15/animals-15-00102-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/11718982/6f39638dbee5/animals-15-00102-g004.jpg

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