Ramzan Fahad, Younis Adnan, Lim Ki-Byung
Department of Horticulture, Kyungpook National University, Daegu 41566, Republic of Korea.
Institute of Horticultural Sciences, University of Agriculture, Faisalabad 38040, Pakistan.
Int J Genomics. 2017;2017:7561909. doi: 10.1155/2017/7561909. Epub 2017 Mar 28.
Molecular cytogenetic techniques, such as in situ hybridization methods, are admirable tools to analyze the genomic structure and function, chromosome constituents, recombination patterns, alien gene introgression, genome evolution, aneuploidy, and polyploidy and also genome constitution visualization and chromosome discrimination from different genomes in allopolyploids of various horticultural crops. Using GISH advancement as multicolor detection is a significant approach to analyze the small and numerous chromosomes in fruit species, for example, hybrids. This analytical technique has proved to be the most exact and effective way for hybrid status confirmation and helps remarkably to distinguish donor parental genomes in hybrids such as , , and ornamental hybrids. The genome characterization facilitates in hybrid selection having potential desirable characteristics during the early hybridization breeding, as this technique expedites to detect introgressed sequence chromosomes. This review study epitomizes applications and advancements of genomic in situ hybridization (GISH) techniques in horticultural plants.
分子细胞遗传学技术，如原位杂交方法，是分析基因组结构与功能、染色体组成、重组模式、外源基因渗入、基因组进化、非整倍体和多倍体，以及在各种园艺作物的异源多倍体中可视化基因组组成和区分不同基因组染色体的理想工具。利用基因组原位杂交（GISH）进展进行多色检测是分析水果物种（如杂种）中微小且数量众多的染色体的重要方法。这种分析技术已被证明是确认杂种状态最准确、有效的方法，并且极大地有助于区分杂种（如观赏杂种）中的供体亲本基因组。基因组特征分析有助于在早期杂交育种过程中选择具有潜在理想特性的杂种，因为该技术能快速检测渗入序列染色体。本综述研究概括了基因组原位杂交（GISH）技术在园艺植物中的应用和进展。
