Department of Chemistry and Life Science, BioScience Institute, Sahmyook University, Seoul 01795, Korea.
BioBreeding Institute, Ansung 17544, Korea.
Cells. 2021 Sep 8;10(9):2358. doi: 10.3390/cells10092358.
Intergeneric crosses between species and have produced crops with prominent shoot and root systems of and , respectively. It is necessary to discriminate donor genomes when studying cytogenetic stability in distant crosses to identify homologous chromosome pairing, and microsatellite repeats have been used to discriminate subgenomes in allopolyploids. To identify genome-specific microsatellites, we explored the microsatellite content in three species (, AA, , CC, and , BB) and (RR) genomes, and validated their genome specificity by fluorescence in situ hybridization. We identified three microsatellites showing A, C, and B/R genome specificity. ACBR_msat14 and ACBR_msat20 were detected in the A and C chromosomes, respectively, and ACBR_msat01 was detected in B and R genomes. However, we did not find a microsatellite that discriminated the B and R genomes. The localization of ACBR_msat20 in the 45S rDNA array in × 977 corroborated the association of the 45S rDNA array with genome rearrangement. Along with the rDNA and telomeric repeat probes, these microsatellites enabled the easy identification of homologous chromosomes. These data demonstrate the utility of microsatellites as probes in identifying subgenomes within closely related and species for the analysis of genetic stability of new synthetic polyploids of these genomes.
种间杂交和种间杂交分别产生了具有显著茎和根系的作物，和，分别。在研究远缘杂交中的细胞遗传学稳定性时，有必要区分供体基因组，以识别同源染色体配对，微卫星重复已被用于在异源多倍体中区分亚基因组。为了鉴定基因组特异性微卫星，我们探索了三个种（，AA，，CC，和，BB）和（RR）基因组中的微卫星含量，并通过荧光原位杂交验证了它们的基因组特异性。我们鉴定了三个显示 A、C 和 B/R 基因组特异性的微卫星。ACBR_msat14 和 ACBR_msat20 分别检测到 A 和 C 染色体中，而 ACBR_msat01 检测到 B 和 R 基因组中。然而，我们没有发现一个能够区分 B 和 R 基因组的微卫星。ACBR_msat20 在 45S rDNA 阵列中的定位与 45S rDNA 阵列与基因组重排的关联相吻合。这些微卫星与 rDNA 和端粒重复探针一起，可轻松识别同源染色体。这些数据表明，微卫星作为探针在鉴定和种内紧密相关的种内亚基因组方面具有实用性，可用于分析这些基因组新合成多倍体的遗传稳定性。
