Liu Yong, Liu Honggen, Luo Xuegang, Deng Jingyu, Pan Yuan, Liang Han
Department of Gastric Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer; Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China.
Tumour Biol. 2015 Jun;36(6):4377-86. doi: 10.1007/s13277-015-3077-z. Epub 2015 Jan 28.
SET and MYND domain-containing protein 3 (SMYD3) plays a key role in the progression of human cancer. Matrix metalloproteinase (MMP)-9 is being related to tumor progression. It has been reported that SMYD3 and MMP-9 are overexpressed in human cancers. However, the exact roles of SMYD3 and MMP-9 in the metastasis and prognosis of gastric cancer (GC) remain unclear. The expressions of SMYD3 and MMP-9 were detected by semiquantitative reverse transcription polymerase chain reaction and Western blotting in gastric cancer and adjacent nontumor tissues. In addition, SMYD3 and MMP-9 expressions were analyzed by immunohistochemistry in formalin-fixed samples from 186 gastric cancer patients. The messenger RNA (mRNA) and protein expression levels of SMYD3 and MMP-9 in gastric cancer tissues were both significantly higher than those in adjacent nontumor tissues. In addition, the expression of SMYD3 was correlated with size of primary tumor and lymph node metastasis, while size of primary tumor and serosal invasion were identified as the independently relative factors of MMP-9 expression in GC tissues. SMYD3 expression and MMP-9 expression in GC tissues were significantly and positively correlated. Multivariate analysis results demonstrated that degree of differentiation, lymph node metastasis, TNM stage, SMYD3 expression, and MMP-9 expression were the independent prognostic indicators of gastric cancer. SMYD3 and MMP-9 may play important roles in tumor invasion, metastasis, and prognosis and could work as promising targets for prognostic prediction in gastric cancer.
含SET和MYND结构域蛋白3（SMYD3）在人类癌症进展中起关键作用。基质金属蛋白酶（MMP）-9与肿瘤进展有关。据报道，SMYD3和MMP-9在人类癌症中过表达。然而，SMYD3和MMP-9在胃癌（GC）转移和预后中的确切作用仍不清楚。采用半定量逆转录聚合酶链反应和蛋白质印迹法检测胃癌组织及癌旁非肿瘤组织中SMYD3和MMP-9的表达。此外，通过免疫组织化学分析186例胃癌患者福尔马林固定样本中的SMYD3和MMP-9表达。胃癌组织中SMYD3和MMP-9的信使核糖核酸（mRNA）和蛋白表达水平均显著高于癌旁非肿瘤组织。此外，SMYD3的表达与原发肿瘤大小和淋巴结转移相关，而原发肿瘤大小和浆膜侵犯被确定为GC组织中MMP-9表达的独立相关因素。GC组织中SMYD3表达与MMP-9表达显著正相关。多因素分析结果显示，分化程度、淋巴结转移、TNM分期、SMYD3表达和MMP-9表达是胃癌的独立预后指标。SMYD3和MMP-9可能在肿瘤侵袭、转移和预后中起重要作用，并且可能成为胃癌预后预测的有前景的靶点。
