关于谷胱甘肽还原铬酸盐的机制：谷胱甘肽自由基和可分离的Cr(V)中间体的电子顺磁共振证据。
On the mechanism of the chromate reduction by glutathione: ESR evidence for the glutathionyl radical and an isolable Cr(V) intermediate.
作者信息
Shi X L, Dalal N S
机构信息
Department of Chemistry, West Virginia University, Morgantown 26506.
出版信息
Biochem Biophys Res Commun. 1988 Oct 14;156(1):137-42. doi: 10.1016/s0006-291x(88)80815-5.
PMID:2845969
Abstract
With a view of elucidating the role of glutathione (GSH) in the biochemical pathways of the chromate-exposure related carcinogenesis, we carried out electron spin resonance (ESR) spectroscopic investigations of the chromate-GSH redox reactions. The ESR measurements, employing spin-traps, provide evidence for the involvement of the glutathione (GS) radical, as well as an isolable Cr(V)-glutathione intermediate. These results indicate a new mechanism for the reduction of chromate by GSH in in vitro cellular environment and help understand the (unexpected) increase in Cr(VI)-induced DNA strand breaks at elevated GSH levels.
摘要
为了阐明谷胱甘肽（GSH）在铬酸盐暴露相关致癌生化途径中的作用，我们对铬酸盐 - GSH氧化还原反应进行了电子自旋共振（ESR）光谱研究。采用自旋捕获剂的ESR测量为谷胱甘肽（GS）自由基以及可分离的Cr（V） - 谷胱甘肽中间体的参与提供了证据。这些结果表明了在体外细胞环境中GSH还原铬酸盐的新机制，并有助于理解在GSH水平升高时Cr（VI）诱导的DNA链断裂（意外）增加的现象。
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