Özay Rafet, Türkoğlu Mehmet Erhan, Gürer Bora, Dolgun Habibullah, Evirgen Oya, Ergüder Berrin İmge, Hayırlı Nazlı, Gürses Levent, Şekerci Zeki
Ministry of Health Diskapi Yildirim Beyazit Training and Research Hospital, Neurosurgery Clinic, Ankara, Turkey.
Ministry of Health Diskapi Yildirim Beyazit Training and Research Hospital, Neurosurgery Clinic, Ankara, Turkey.
World Neurosurg. 2017 Aug;104:634-643. doi: 10.1016/j.wneu.2017.04.136. Epub 2017 Apr 28.
The development of secondary brain injury via oxidative stress after traumatic brain injury (TBI) is a well-known entity. Consequently, the aim of the present study was to evaluate the role of omeprazole (OM) on rat model of TBI.
A total of 24 male rats were used and divided into 4 groups as follows; control, trauma, OM, and methylprednisolone (MP). The trauma, OM, and MP groups were subjected to closed-head contusive weight-drop injuries. Rats received treatment with saline, OM, or MP, respectively. All the animals were sacrificed at 24 hours after trauma and brain tissues were extracted. The oxidant/antioxidant parameters (malondialdehyde, glutathione peroxidase, superoxide dismutase, nitric oxide) and caspase-3 in the cerebral tissue were analyzed, and histomorphologic evaluation of the cerebral tissue was performed.
Levels of MDA and activity of caspase-3 were significantly reduced in the OM and MP groups compared with the trauma group. Glutathione peroxidase and superoxide dismutase levels were increased both in the OM and MP groups compared with the trauma group. The pathology scores were statistically lower in the OM and MP groups than the trauma group.
The results of the present study showed that OM was as effective as MP in protecting brain from oxidative stress, and apoptosis in the early phase of TBI.
创伤性脑损伤（TBI）后通过氧化应激导致的继发性脑损伤的发展是一个众所周知的现象。因此，本研究的目的是评估奥美拉唑（OM）在TBI大鼠模型中的作用。
总共使用24只雄性大鼠，分为以下4组：对照组、创伤组、OM组和甲基强的松龙（MP）组。创伤组、OM组和MP组接受闭合性颅脑撞击重物下落损伤。大鼠分别接受生理盐水、OM或MP治疗。所有动物在创伤后24小时处死并提取脑组织。分析脑组织中的氧化/抗氧化参数（丙二醛、谷胱甘肽过氧化物酶、超氧化物歧化酶、一氧化氮）和半胱天冬酶-3，并对脑组织进行组织形态学评估。
与创伤组相比，OM组和MP组的丙二醛水平和半胱天冬酶-3活性显著降低。与创伤组相比，OM组和MP组的谷胱甘肽过氧化物酶和超氧化物歧化酶水平均升高。OM组和MP组的病理评分在统计学上低于创伤组。
本研究结果表明，OM在保护脑免受氧化应激和TBI早期凋亡方面与MP一样有效。
