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一个过渡增殖群体支撑着睾丸高效的再生能力。

A transit-amplifying population underpins the efficient regenerative capacity of the testis.

作者信息

Carrieri Claudia, Comazzetto Stefano, Grover Amit, Morgan Marcos, Buness Andreas, Nerlov Claus, O'Carroll Dónal

机构信息

MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH16 4UU, Scotland, UK.

European Molecular Biology Laboratory, Mouse Biology Unit, Monterotondo Scalo 00015, Italy.

出版信息

J Exp Med. 2017 Jun 5;214(6):1631-1641. doi: 10.1084/jem.20161371. Epub 2017 May 1.

DOI:10.1084/jem.20161371
PMID:28461596
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5460999/
Abstract

The spermatogonial stem cell (SSC) that supports spermatogenesis throughout adult life resides within the GFRα1-expressing A type undifferentiated spermatogonia. The decision to commit to spermatogenic differentiation coincides with the loss of GFRα1 and reciprocal gain of Ngn3 (Neurog3) expression. Through the analysis of the piRNA factor (), we identify a novel population of Ngn3-expressing spermatogonia that are essential for efficient testicular regeneration after injury. Depletion of -expressing cells results in a transient impact on testicular homeostasis, with this population behaving strictly as transit amplifying cells under homeostatic conditions. However, upon injury, -expressing cells are essential for the efficient regenerative capacity of the testis, and also display facultative stem activity in transplantation assays. In summary, the mouse testis has adopted a regenerative strategy to expand stem cell activity by incorporating a transit-amplifying population to the effective stem cell pool, thus ensuring rapid and efficient tissue repair.

摘要

支持成年期全程精子发生的精原干细胞（SSC）存在于表达胶质细胞源性神经营养因子受体α1（GFRα1）的A型未分化精原细胞中。决定进行生精分化与GFRα1的丧失以及神经生成素3（Neurog3，Ngn3）表达的相应增加同时发生。通过对piRNA因子的分析，我们鉴定出了一群新的表达Ngn3的精原细胞，它们对于损伤后睾丸的有效再生至关重要。表达该因子的细胞耗竭会对睾丸内环境稳定产生短暂影响，在稳态条件下，这群细胞严格作为过渡增殖细胞发挥作用。然而，在损伤后，表达该因子的细胞对于睾丸的有效再生能力至关重要，并且在移植实验中也表现出兼性干细胞活性。总之，小鼠睾丸采用了一种再生策略，通过将一个过渡增殖群体纳入有效的干细胞库来扩大干细胞活性，从而确保快速而高效的组织修复。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3bc/5460999/754cf3d2814c/JEM_20161371_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3bc/5460999/0801644fe519/JEM_20161371_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3bc/5460999/4b118ec73330/JEM_20161371_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3bc/5460999/cf114dfecdc1/JEM_20161371_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3bc/5460999/754cf3d2814c/JEM_20161371_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3bc/5460999/0801644fe519/JEM_20161371_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3bc/5460999/4b118ec73330/JEM_20161371_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3bc/5460999/cf114dfecdc1/JEM_20161371_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3bc/5460999/754cf3d2814c/JEM_20161371_Fig4.jpg

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