Hill T M, Pelletier A J, Tecklenburg M L, Kuempel P L
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309.
Cell. 1988 Nov 4;55(3):459-66. doi: 10.1016/0092-8674(88)90032-3.
The terminus region of the E. coli chromosome contains two loci, T1 and T2, that inhibit the progress of replication forks and require the trans-acting factor tus. We have identified a 23 bp terminator signal at T1 and T2 that is within 100 bp of the sites of replication arrest. When an oligodeoxyribonucleotide containing the terminator signal was inserted into a plasmid, replication was halted only in a tus+ strain and when the terminator signal was oriented properly. We also found this terminator sequence in the terminus region of the plasmid R6K and in the origin region of RepFIIA class plasmids. In addition, we found striking similarities between the E. coli terminator signal and the terminator sequence of B. subtilis.
大肠杆菌染色体的末端区域包含两个位点，T1和T2，它们会抑制复制叉的前进，并且需要反式作用因子tus。我们在T1和T2处鉴定出一个23 bp的终止信号，该信号位于复制停滞位点的100 bp范围内。当将含有终止信号的寡脱氧核糖核苷酸插入质粒时，复制仅在tus+菌株中且终止信号方向正确时才会停止。我们还在质粒R6K的末端区域和RepFIIA类质粒的起源区域中发现了这个终止序列。此外，我们发现大肠杆菌终止信号与枯草芽孢杆菌的终止序列之间存在显著相似性。
