固定细胞学标本的老化会导致染色体DNA降解。
Ageing of fixed cytological preparations produces degradation of chromosomal DNA.
作者信息
Mezzanotte R, Vanni R, Flore O, Ferrucci L, Sumner A T
机构信息
Istituto di Biologia Generale, Facolta di Medicina e Chirurgia, Universita di Cagliari, Italy.
出版信息
Cytogenet Cell Genet. 1988;48(1):60-2. doi: 10.1159/000132588.
When fixed chromosome preparations were allowed to age for 1-72 h, they became progressively more susceptible to digestion by exonuclease III and by S1 nuclease. Analysis of DNA from these aged preparations on agarose gels showed that the molecular weight of the DNA decreased as ageing progressed. We conclude that DNA in fixed chromosome preparations becomes progressively degraded as the preparations age.
当固定后的染色体标本放置1至72小时使其老化时，它们对外切核酸酶III和S1核酸酶的消化作用变得越来越敏感。对这些老化标本的DNA在琼脂糖凝胶上进行分析，结果显示随着老化过程的推进，DNA的分子量逐渐降低。我们得出结论，固定后的染色体标本中的DNA会随着标本的老化而逐渐降解。
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