Abbaszadegan Mohammad Reza, Moghbeli Meysam
Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Medical Genetics Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.
Pathol Oncol Res. 2018 Apr;24(2):245-250. doi: 10.1007/s12253-017-0243-1. Epub 2017 May 1.
Homeobox (HOX) transcription factors and NOTCH signaling pathway are critical regulators of stem cell functions, cell fate in development and homeostasis of gastrointestinal tissues. In the present study, we analyzed cross talk between NOTCH pathway and HOX genes through assessment of probable correlation betweenMAML1 and MEIS1 as the main transcription factor of NOTCH pathway and enhancer of HOX transcriptional machinery, respectively in esophageal squamous cell carcinoma (ESCC) patients. Fifty one ESCC cases were enrolled to assess the levels of Meis1 and Maml1 mRNA expression using real-time polymerase chain reaction (PCR). Only 3 out of 51 (5.9%) cases had MEIS1/MAML1 under expression and 2/51 (3.9%) cases had MEIS1/MAML1over expression. Nine out of 51 samples (17.6%) have shown MEIS1 under expression and MAML1 over expression. There was a significant correlation between MAML1and MEIS1mRNA expressions (p ≤ 0.05). There were significant correlations between MEIS1 under/MAML1 over expressed cases and tumor location (p = 0.05), tumor depth of invasion (p = 0.011), and sex (p = 0.04). Our results showed that MEIS1 may have a negative role in regulation of MAML1expression during the ESCC progression.
同源框（HOX）转录因子和NOTCH信号通路是干细胞功能、发育过程中的细胞命运以及胃肠道组织稳态的关键调节因子。在本研究中，我们通过评估MAML1和MEIS1之间可能的相关性，分析了NOTCH通路与HOX基因之间的相互作用，MAML1和MEIS1分别是NOTCH通路的主要转录因子和HOX转录机制的增强子，研究对象为食管鳞状细胞癌（ESCC）患者。纳入51例ESCC病例，采用实时聚合酶链反应（PCR）评估Meis1和Maml1 mRNA表达水平。51例病例中只有3例（5.9%）MEIS1/MAML1表达不足，2例（3.9%）MEIS1/MAML1表达过度。51个样本中有9例（17.6%）显示MEIS1表达不足而MAML1表达过度。MAML1和MEIS1 mRNA表达之间存在显著相关性（p≤0.05）。MEIS1表达不足/MAML1表达过度的病例与肿瘤位置（p = 0.05）、肿瘤浸润深度（p = 0.011）和性别（p = 0.04）之间存在显著相关性。我们的结果表明，在ESCC进展过程中，MEIS1可能在调节MAML1表达方面发挥负向作用。
