Jones Alex R
School of Chemistry, Photon Science Institute and Manchester Institute of Biotechnology, The University of Manchester, Manchester, UK.
Photochem Photobiol Sci. 2017 Jun 14;16(6):820-834. doi: 10.1039/c7pp00054e.
Biologically active derivatives of vitamin B are organometallic cobalt-corrinoid complexes crucial for the healthy function of humans, animals and microbes. Their role as cofactors to numerous, thermally-driven metabolic enzymes is well described, and varies depending on the nature of the upper axial ligand. This ligand also significantly influences the photophysics and photochemistry of B. In this Perspective I will discuss the various aspects of B photochemistry, from its dynamic spin chemistry to the considerable potential it has for biotechnology applications. Although for many years this photochemistry was thought to have no biological function, in recent years it has become apparent that B photochemistry at very least has a role in light-dependent bacterial transcriptional regulation. I will provide an overview of what has been reported about B photobiology to date, with particular emphasis on the mechanism of the transcriptional repressor, CarH, the subject of my Young Investigator Award Plenary Lecture at the European Society for Photobiology Congress 2015 in Aveiro, Portugal.
维生素B的生物活性衍生物是有机金属钴类咕啉配合物，对人类、动物和微生物的健康功能至关重要。它们作为众多热驱动代谢酶的辅助因子的作用已得到充分描述，并且会根据上轴向配体的性质而有所不同。该配体还会显著影响维生素B的光物理和光化学性质。在这篇综述中，我将讨论维生素B光化学的各个方面，从其动态自旋化学到其在生物技术应用中的巨大潜力。尽管多年来人们认为这种光化学没有生物学功能，但近年来很明显，维生素B光化学至少在依赖光的细菌转录调控中发挥作用。我将概述迄今为止关于维生素B光生物学的报道，特别强调转录阻遏物CarH的作用机制，这是我在2015年于葡萄牙阿威罗举行的欧洲光生物学大会上获得青年研究者奖全会演讲的主题。
