Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Bld. 9, 1113 Sofia, Bulgaria.
Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Bld. 25, 1113 Sofia, Bulgaria.
Int J Mol Sci. 2023 Feb 23;24(5):4400. doi: 10.3390/ijms24054400.
Photodynamic therapy (PDT) is a curative method, firstly developed for cancer therapy with fast response after treatment and minimum side effects. Two zinc(II) phthalocyanines (3ZnPc and 4ZnPc) and a hydroxycobalamin (Cbl) were investigated on two breast cancer cell lines (MDA-MB-231 and MCF-7) in comparison to normal cell lines (MCF-10 and BALB 3T3). The novelty of this study is a complex of non-peripherally methylpyridiloxy substituted Zn(II) phthalocyanine (3ZnPc) and the evaluation of the effects on different cell lines due to the addition of second porphyrinoid such as Cbl. The results showed the complete photocytotoxicity of both ZnPc-complexes at lower concentrations (<0.1 μM) for 3ZnPc. The addition of Cbl caused a higher phototoxicity of 3ZnPc at one order lower concentrations (<0.01 μM) with a diminishment of the dark toxicity. Moreover, it was determined that an increase of the selectivity index of 3ZnPc, from 0.66 (MCF-7) and 0.89 (MDA-MB-231) to 1.56 and 2.31, occurred by the addition of Cbl upon exposure with a LED 660 nm (50 J/cm). The study suggested that the addition of Cbl can minimize the dark toxicity and improve the efficiency of the phthalocyanines for anticancer PDT applications.
光动力疗法（PDT）是一种治疗方法，最初是为癌症治疗开发的，具有治疗后快速反应和最小副作用的特点。两种锌(II)酞菁（3ZnPc 和 4ZnPc）和一个羟钴胺素（Cbl）被用于两种乳腺癌细胞系（MDA-MB-231 和 MCF-7）与正常细胞系（MCF-10 和 BALB 3T3）进行比较。这项研究的新颖之处在于非外周甲基吡啶氧基取代的锌(II)酞菁（3ZnPc）的复合物，并评估了由于添加第二卟啉类物质如 Cbl 对不同细胞系的影响。结果表明，两种 ZnPc 配合物在较低浓度（<0.1 μM）下对 3ZnPc 具有完全的光细胞毒性。Cbl 的添加导致 3ZnPc 的光毒性增加一个数量级，在低至<0.01 μM 的浓度下，暗毒性降低。此外，通过在 660nm LED（50 J/cm）照射下添加 Cbl，确定 3ZnPc 的选择性指数从 0.66（MCF-7）和 0.89（MDA-MB-231）分别增加到 1.56 和 2.31。研究表明，添加 Cbl 可以最小化暗毒性并提高酞菁用于抗癌 PDT 应用的效率。
