Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Dermatology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
J Oral Pathol Med. 2017 Nov;46(10):1023-1029. doi: 10.1111/jop.12588. Epub 2017 May 25.
There is a reported relation between hyperhomocysteinemia and lichen planus (LP). An increase in homocysteine (Hcy) and the risk of cardiovascular disease (CVD) in patients with methylenetetrahydrofolate reductase (MTHFR) mutation has been described.
To detect MTHFR (C677T) gene polymorphism, and to find its association with CVD risk, Hcy and folic acid levels in patients with LP.
This hospital-based case-control study included 110 patients with LP: 70 with cutaneous LP (CLP) and 40 with oral LP (OLP). A total of 120 age- and sex-matched healthy subjects were used as controls. Three millilitre venous blood sample was taken for detection of MTHFR gene polymorphism by PCR-RFLP technique and for measurement of the lipid profile. Hcy and folic acid were measured by ELISA. Hypertension was evaluated.
There were significantly higher prevalence of hypertension with higher Hcy, triglycerides and cholesterol levels and lower folic acid and HDL levels among patients' groups. Hypertension with higher Hcy and cholesterol levels together with lower folic acid and HDL levels have been found in OLP when compared to CLP. Patients showed a significant higher percentage of the MTHFR 677 TT genotype (P=.003) and of the MTHFR 677 T allele (P=.042) compared to controls. Moreover, there was a higher prevalence of MTHFR 677 T allele in patients with CLP.
MTHFR 677 gene polymorphism may be a risk factor for the development of the LP, and to predispose these patients to higher risk of CVD.
高同型半胱氨酸血症与扁平苔藓（LP）之间存在报告关系。已描述亚甲基四氢叶酸还原酶（MTHFR）突变患者同型半胱氨酸（Hcy）增加和心血管疾病（CVD）的风险。
检测 MTHFR（C677T）基因多态性，并发现其与 LP 患者 CVD 风险、Hcy 和叶酸水平的关系。
本基于医院的病例对照研究纳入了 110 例 LP 患者：70 例皮肤 LP（CLP）和 40 例口腔 LP（OLP）。共纳入 120 名年龄和性别匹配的健康对照者作为对照组。采集 3ml 静脉血样，采用 PCR-RFLP 技术检测 MTHFR 基因多态性，并检测血脂谱。采用 ELISA 法检测 Hcy 和叶酸。评估高血压。
患者组中高血压的患病率明显较高，伴有较高的 Hcy、甘油三酯和胆固醇水平以及较低的叶酸和 HDL 水平。与 CLP 相比，OLP 中高血压患者伴有更高的 Hcy 和胆固醇水平，以及较低的叶酸和 HDL 水平。与对照组相比，患者的 MTHFR 677 TT 基因型（P=.003）和 MTHFR 677 T 等位基因（P=.042）的比例显著更高。此外，CLP 患者中 MTHFR 677 T 等位基因的患病率更高。
MTHFR 677 基因多态性可能是 LP 发生的危险因素，并使这些患者更容易发生 CVD。
