过敏性哮喘儿童中Th17/Treg失调与Notch表达升高有关。
Th17/Treg dysregulation in allergic asthmatic children is associated with elevated notch expression.
作者信息
Li Changchong, Sheng Anqun, Jia Xiaoxiao, Zeng Zeyu, Zhang Xueya, Zhao Wei, Zhang Weixi
机构信息
a Department of Pediatric Allergy and Immunology , The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University , Wenzhou , Zhejiang Province , China.
b Division of Allergy and Immunology, Department of Pediatrics , Virginia Commonwealth University , Richmond , VA , USA.
出版信息
J Asthma. 2018 Jan;55(1):1-7. doi: 10.1080/02770903.2016.1266494. Epub 2017 May 16.
BACKGROUND
Notch signaling pathway is critically involved in the differentiation of T helper (Th) cells, key players in the pathogenesis of allergic diseases.
OBJECTIVE
The study is to explore whether Th17/Treg dysregulation in children with allergic asthma (AA) is associated with alteration of Notch expression.
METHODS
Thirty-five patients with AA and thirty-five healthy control children were selected. Flow cytometry was used to detect Th17 and Treg cells. Quantitative real-time polymerase chain reaction (QRT-PCR) was used to measure the expression of Notch1 mRNA. The correlations among Notch1 mRNA expression, the percentage of Th17 cells, and Th17/Treg ratio were calculated.
RESULTS
Th17 and Treg cells were significantly increased and decreased, respectively, in children with AA than in healthy control (p < 0.001). mRNA level of Notch1 was elevated in children with AA comparing to healthy controls (p < 0.001). The mRNA expression of Notch1 was positively correlated with the percentage of Th17 cells (r = 0.775, p < 0.001) and Th17/Treg ratio (r = 0.698, p < 0.001).
CONCLUSION
Children with AA showed dysregulation of Th17/Treg cells in peripheral blood. Such change is accompanied with overexpression of Notch1, indicating Th17/Treg dysregulation in children with AA is associated with elevated Notch expression.
背景
Notch信号通路在T辅助（Th）细胞分化中起关键作用，而Th细胞是过敏性疾病发病机制中的关键参与者。
目的
探讨过敏性哮喘（AA）患儿Th17/Treg细胞失调是否与Notch表达改变有关。
方法
选取35例AA患儿和35例健康对照儿童。采用流式细胞术检测Th17和Treg细胞。运用定量实时聚合酶链反应（QRT-PCR）检测Notch1 mRNA的表达。计算Notch1 mRNA表达、Th17细胞百分比和Th17/Treg比值之间的相关性。
结果
与健康对照相比，AA患儿的Th17细胞显著增多，Treg细胞显著减少（p < 0.001）。与健康对照相比，AA患儿的Notch1 mRNA水平升高（p < 0.001）。Notch1的mRNA表达与Th17细胞百分比（r = 0.775，p < 0.001）和Th17/Treg比值（r = 0.698，p < 0.001）呈正相关。
结论
AA患儿外周血Th17/Treg细胞失调。这种变化伴随着Notch1的过表达，表明AA患儿Th17/Treg失调与Notch表达升高有关。
Zotero 插件