Ravikumar K, Boda Sunil Kumar, Basu Bikramjit
Laboratory for Biomaterials, Materials Research Centre, Indian Institute of Science, Bangalore 560012, India.
Laboratory for Biomaterials, Materials Research Centre, Indian Institute of Science, Bangalore 560012, India; Centre for BioSystems Science and Engineering, Indian Institute of Science, Bangalore 560012, India.
Bioelectrochemistry. 2017 Aug;116:52-64. doi: 10.1016/j.bioelechem.2017.03.004. Epub 2017 Mar 30.
Human Mesenchymal Stem cells (hMSCs) have the unique potential to differentiate into multiple cell types. Depending on the cellular microenvironment (physical and biochemical cues), hMSCs can be directed to differentiate into osteogenic, chondrogenic, myogenic and adipogenic lineages. Among the strategies available to direct stem cell fate processes, electrical stimulation based approach has been extensively investigated in recent studies. In the present study, the conducting Hydroxyapatite-CaTiO (HA-CT) composites are used as electroconductive platforms to support the differentiation of hMSCs, in vitro. During culture without osteogenic supplements, intermittent electrical stimulation is provided every 24h over a period of 4weeks through parallel plate electrodes separated by a distance of 15mm and maintained at a static potential of 15V for 10min. In addition to cell morphological changes, the differentiation behavior of hMSCs after electrical stimulation is evaluated by mRNA expression analysis through polymerase chain reaction (PCR). Importantly, specific bone markers, in particular ALP, Col IA and Osteocalcin are expressed more significantly due to electrical stimulation, which also enhances the extent of extracellular matrix mineralization. Taken together, this study establishes the effectiveness of electroconductive HA-CT composites together with intermittent electrical stimulation to direct osteogenesis of hMSCs.
人骨髓间充质干细胞（hMSCs）具有分化为多种细胞类型的独特潜力。根据细胞微环境（物理和生化信号），hMSCs可被引导分化为成骨、软骨、成肌和脂肪生成谱系。在可用于引导干细胞命运过程的策略中，基于电刺激的方法在最近的研究中得到了广泛研究。在本研究中，导电的羟基磷灰石-CaTiO（HA-CT）复合材料被用作导电平台，以在体外支持hMSCs的分化。在没有成骨补充剂的培养过程中，通过相距15mm的平行板电极，每24小时提供一次间歇性电刺激，持续4周，并在15V的静态电位下保持10分钟。除了细胞形态变化外，通过聚合酶链反应（PCR）进行mRNA表达分析，评估电刺激后hMSCs的分化行为。重要的是，由于电刺激，特定的骨标志物，特别是碱性磷酸酶（ALP）、I型胶原（Col IA）和骨钙素表达更为显著，这也增强了细胞外基质矿化的程度。综上所述，本研究证实了导电HA-CT复合材料与间歇性电刺激共同引导hMSCs成骨的有效性。
