Trace Analysis and Biosensor Research Center, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Department of Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Trace Analysis and Biosensor Research Center, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Department of Applied Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Biosens Bioelectron. 2017 Oct 15;96:84-88. doi: 10.1016/j.bios.2017.04.042. Epub 2017 Apr 27.
A simple renewable surface for a rapid antibacterial susceptibility test has been demonstrated. The 3-aminophenylboronic acid (3-APBA) modified electrode bind with cis-diol groups on the cell wall of both gram positive and gram negative bacteria. The detection of antibacterial susceptibility response by a capacitive system can be done within a short time, 2.5h for the whole process, with good repeatability of the electrode's preparation. An acid solution, could break the bonding between 3-APBA and the bacteria, which were then easily removed by the fluid flow, renewing the sensing surface for the next test. This modified electrode can be reused up to 35 times. This sensor is useful for testing the susceptibility of bacteria to antibacterial agents that affect their cell wall. Results from the capacitive sensor corresponded well with the antimicrobial information in the literature and to the morphology of the treated bacteria revealed by scanning electron microscopy. Antimicrobial susceptibility to natural products could also be easily tested.
已经证明了一种用于快速抗菌药敏测试的简单可再生表面。3-氨苯基硼酸（3-APBA）修饰电极与革兰氏阳性和革兰氏阴性细菌细胞壁上的顺二醇基团结合。通过电容系统检测抗菌药敏反应可以在短时间内完成，整个过程只需 2.5 小时，电极制备的重复性好。酸溶液可以破坏 3-APBA 与细菌之间的结合，然后通过流体流动很容易将其去除，从而为下一次测试更新传感表面。这种修饰电极可以重复使用多达 35 次。该传感器可用于测试对抗菌药物的药敏性，这些药物会影响其细胞壁。电容传感器的结果与文献中的抗菌信息以及扫描电子显微镜显示的处理后细菌的形态非常吻合。天然产物的抗菌药敏性也可以很容易地进行测试。
