Klemes Jan, Kotzianova Adela, Pokorny Marek, Mojzes Peter, Novak Jindrich, Sukova Lada, Demuth Jaroslav, Vesely Jaroslav, Sasek Ladislav, Velebny Vladimir
Contipro a.s., R&D Department, CZ-56102, Dolni Dobrouc, Czech Republic.
Masaryk University, Faculty of Science, Department of Chemistry, CZ-62500, Brno, Czech Republic.
J Biophotonics. 2017 Nov;10(11):1442-1449. doi: 10.1002/jbio.201600284. Epub 2017 May 2.
Non-invasive optical diagnostic methods allow important information about studied systems to be obtained in a non-destructive way. Complete diagnosis requires information about the chemical composition as well as the morphological structure of a sample. We report on the development of an opto-mechanical probe that combines Raman spectroscopy (RS) and optical coherence tomography (OCT), two methods that provide all the crucial information needed for a non-invasive diagnosis. The aim of this paper is to introduce the technical design, construction and optimization of a dual opto-mechanical probe combining two in-house developed devices for confocal RS and OCT. The unique benefit of the probe is a gradual acquisition of OCT and RS data, which allows to use the acquired OCT images to pinpoint locations of interest for RS measurements. The parameters and the correct functioning of the probe were verified by RS scanning of various samples (silicon wafer and ex vivo tissue) based on their OCT images - lateral as well as depth scanning was performed. Both the OCT and RS systems were developed, optimized and tested with the ultimate aim of verifying the functionality of the probe. Picture: Schematic illustration and visualization of the developed RS-OCT probe.
非侵入式光学诊断方法能够以非破坏性的方式获取有关被研究系统的重要信息。完整的诊断需要有关样品化学成分以及形态结构的信息。我们报告了一种光机械探头的研发情况，该探头结合了拉曼光谱（RS）和光学相干断层扫描（OCT）这两种方法，它们能提供非侵入式诊断所需的所有关键信息。本文旨在介绍一种双光机械探头的技术设计、构造和优化，该探头结合了两个内部开发的用于共焦RS和OCT的设备。该探头的独特优势在于可逐步采集OCT和RS数据，这使得能够利用采集到的OCT图像来确定RS测量的感兴趣位置。基于各种样品（硅晶片和离体组织）的OCT图像，通过RS扫描对探头的参数和正确功能进行了验证——进行了横向以及深度扫描。开发、优化和测试OCT和RS系统的最终目的是验证探头的功能。图：所开发的RS - OCT探头的示意图及可视化图。
