拉曼光谱-光谱域光学相干断层成像集成系统。
Integrated system for combined Raman spectroscopy-spectral domain optical coherence tomography.
机构信息
Vanderbilt University, Department of Biomedical Engineering, Station B, Box 351631, Nashville, Tennessee 37235, USA.
出版信息
J Biomed Opt. 2011 Jan-Feb;16(1):011007. doi: 10.1117/1.3520132.
Abstract
Raman spectroscopy (RS) and optical coherence tomography (OCT) are powerful tools for optical analysis of tissues with mutually complementary strengths and limitations. OCT excels at visualizing tissue microstructure but lacks molecular specificity, while RS can relay tissue biochemical composition but typically cannot relate microstructure. Previous implementations of combined RS-OCT have utilized a common sample arm while maintaining independent RS and OCT detection arms. We present the design and application of an integrated RS-OCT instrument with a common detection arm for both RS and OCT. The detector is a spectrograph capable of sequential detection of the 855-nm OCT signal and the Raman scatter generated by a 785-nm source. The capabilities of the instrument are demonstrated ex vivo in the calvaria and retina of rodents, as well as in vivo in human skin.
摘要
拉曼光谱（RS）和光相干断层扫描（OCT）是用于分析组织的强大光学工具，具有相互补充的优势和局限性。OCT 在可视化组织微观结构方面表现出色，但缺乏分子特异性，而 RS 可以传递组织生化组成，但通常无法关联微观结构。以前的组合 RS-OCT 实现方法利用了共同的样本臂，同时保持了独立的 RS 和 OCT 检测臂。我们提出了一种具有共同检测臂的集成 RS-OCT 仪器的设计和应用，用于 RS 和 OCT 的检测。该探测器是一个光谱仪，能够顺序检测 855nm 的 OCT 信号和 785nm 光源产生的拉曼散射。该仪器的功能在啮齿动物的颅骨和视网膜以及人体皮肤的体内和体外得到了验证。
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