Wagnerova Alexandra, Babickova Janka, Liptak Robert, Vlkova Barbora, Celec Peter, Gardlik Roman
Faculty of Medicine, Institute of Molecular Biomedicine, Comenius University, 811 08 Bratislava, Slovakia.
Faculty of Medicine, Institute of Medical Physics, Biophysics, Informatics and Telemedicine, Comenius University, 811 08 Bratislava, Slovakia.
Gastroenterol Res Pract. 2017;2017:8051870. doi: 10.1155/2017/8051870. Epub 2017 Mar 29.
Resveratrol is a natural polyphenol studied for its possible protective properties in inflammatory bowel diseases. Moreover, it has been shown to interact with estrogen receptors. In the present study, we aimed to investigate possible diverse effects of resveratrol on female and male mice in DSS-induced colitis. Thirty-seven C57BL/6 mice (21 female and 16 male) were divided into three groups for each sex. The first group received pure water (CTRL). The other two groups received 1.5% dextran sulfate sodium (DSS) to induce colitis from which one group was treated with resveratrol (DSS + RSV). Intake of 1.5% DSS caused weight loss in all DSS groups compared to control mice. Weight loss, stool consistency, and discomfort did not show any protective effect of resveratrol in males and showed even adverse effects in females. In females, the activity of myeloperoxidase was lower compared to that in males. However, colon length and spleen weight showed no sex differences, which can indicate the induction of only mild colitis in mice. Resveratrol did not have any effect on TNF-alpha levels. Taken together, these results for the first time propose possible diverse effects of resveratrol in DSS-induced colitis model depending on the sex of the animal. However, this conclusion must be confirmed by further analyses.
白藜芦醇是一种天然多酚，因其在炎症性肠病中可能具有的保护特性而受到研究。此外，它已被证明能与雌激素受体相互作用。在本研究中，我们旨在调查白藜芦醇对葡聚糖硫酸钠（DSS）诱导的结肠炎模型中雌性和雄性小鼠可能产生的不同影响。将37只C57BL/6小鼠（21只雌性和16只雄性）按性别分为三组。第一组给予纯水（对照组）。另外两组给予1.5%的葡聚糖硫酸钠以诱导结肠炎，其中一组用白藜芦醇治疗（DSS + RSV）。与对照小鼠相比，摄入1.5%的DSS导致所有DSS组体重减轻。体重减轻、粪便稠度和不适情况表明，白藜芦醇对雄性小鼠没有任何保护作用，对雌性小鼠甚至有不良影响。雌性小鼠中髓过氧化物酶的活性低于雄性小鼠。然而，结肠长度和脾脏重量没有性别差异，这可能表明小鼠仅诱发了轻度结肠炎。白藜芦醇对肿瘤坏死因子-α水平没有任何影响。综上所述，这些结果首次表明，在DSS诱导的结肠炎模型中，白藜芦醇根据动物性别可能产生不同影响。然而，这一结论必须通过进一步分析来证实。
