Musiani M, Zerbini M, Gentilomi G, La Placa M
Institute of Microbiology, University of Bologna, Italy.
J Virol Methods. 1988 Aug;20(4):333-40. doi: 10.1016/0166-0934(88)90136-x.
A rapid quantitative assay was developed to determine cytomegalovirus infectious units. The assay yielded results in three days. The rapid quantitation of cytomegalovirus infectivity was developed by evaluating the number of cells that expressed cytomegalovirus-late antigens at the end of a single replication cycle (62 h post-infection). Late antigens were visualized by immunoalkaline phosphatase staining using a monoclonal antibody against the major viral capsid protein (66.8 kDa). Thirty-five cytomegalovirus samples were examined. The values of the infectious units obtained by immunoalkaline phosphatase staining were correlated significantly with the values determined by the classical plaque-forming technique.
开发了一种快速定量测定法来确定巨细胞病毒感染单位。该测定法在三天内得出结果。通过评估在单个复制周期结束时（感染后62小时）表达巨细胞病毒晚期抗原的细胞数量，开发了巨细胞病毒感染性的快速定量方法。使用针对主要病毒衣壳蛋白（66.8 kDa）的单克隆抗体，通过免疫碱性磷酸酶染色使晚期抗原可视化。检查了35个巨细胞病毒样本。通过免疫碱性磷酸酶染色获得的感染单位值与通过经典噬斑形成技术确定的值显著相关。
