Musiani M, Zerbini M, La Placa M
J Immunol Methods. 1986 Apr 17;88(2):255-8. doi: 10.1016/0022-1759(86)90013-x.
The use of alkaline phosphatase immunocytochemical staining was explored for the rapid diagnosis of poliovirus, adenovirus, herpes simplex virus and cytomegalovirus infections in cell cultures. In this test, viral antigens treated with their relative antibody were incubated with alkaline phosphatase-labelled antisera. The enzyme label was developed with a naphthol salt in the presence of a diazonium salt (Fast Blue) in order to obtain a blue coloured precipitate at the site of the enzyme. It is suggested that this immunocytochemical technique is valuable in the detection of viral infections and would be an appropriate test to use when rapid diagnosis is required.
探索了使用碱性磷酸酶免疫细胞化学染色法对细胞培养中的脊髓灰质炎病毒、腺病毒、单纯疱疹病毒和巨细胞病毒感染进行快速诊断。在该试验中，用其相应抗体处理过的病毒抗原与碱性磷酸酶标记的抗血清一起孵育。酶标记物在重氮盐（固蓝）存在的情况下用萘酚盐显色，以便在酶所在部位获得蓝色沉淀。有人认为，这种免疫细胞化学技术在病毒感染检测中很有价值，并且在需要快速诊断时是一种合适的检测方法。
