利用针对病毒主要立即早期蛋白的单克隆抗体对巨细胞病毒进行快速定量及中和抗体检测。
Rapid quantitation of cytomegalovirus and assay of neutralizing antibody by using monoclonal antibody to the major immediate-early viral protein.
作者信息
Chou S W, Scott K M
机构信息
Medical Service, Veterans Administration Medical Center, Portland, Oregon.
出版信息
J Clin Microbiol. 1988 Mar;26(3):504-7. doi: 10.1128/jcm.26.3.504-507.1988.
Abstract
An overnight assay, based on staining cytomegalovirus-infected cells with monoclonal antibody to the 72,000-molecular-weight major immediate-early viral protein, was compared with a conventional 14-day plaque assay for quantitation of cell-free stocks of cytomegalovirus laboratory strain AD-169 and 20 other clinical strains. Viral titers were quantitatively similar when determined by either method, but centrifugation of monolayers during inoculation enhanced viral infectivity an average of 4.1-fold. When used for scoring neutralizing antibody assays, monoclonal antibody staining yielded titers within one dilution of 14-day plaque-reduction assays in 54 of 56 titrations. Of 21 cytomegalovirus strains, 2 were not recognized by the monoclonal antibody used. Assay with monoclonal antibody offers a rapid and accurate alternative to plaque assays for quantitation or neutralization of cytomegalovirus.
摘要
基于用针对分子量为72,000的主要立即早期病毒蛋白的单克隆抗体对巨细胞病毒感染细胞进行染色的过夜检测法，与用于定量巨细胞病毒实验室毒株AD - 169和其他20种临床毒株的无细胞毒株的传统14天蚀斑检测法进行了比较。通过任一种方法测定时，病毒滴度在数量上相似，但接种期间单层细胞的离心平均使病毒感染性提高了4.1倍。当用于中和抗体检测的评分时，在56次滴定中的54次中，单克隆抗体染色产生的滴度与14天蚀斑减少检测法的滴度相差不超过一个稀释度。在21种巨细胞病毒毒株中，有2种未被所用的单克隆抗体识别。用单克隆抗体进行检测为巨细胞病毒的定量或中和提供了一种快速且准确的替代蚀斑检测法的方法。
相似文献
1
Rapid quantitation of cytomegalovirus and assay of neutralizing antibody by using monoclonal antibody to the major immediate-early viral protein.利用针对病毒主要立即早期蛋白的单克隆抗体对巨细胞病毒进行快速定量及中和抗体检测。
J Clin Microbiol. 1988 Mar;26(3):504-7. doi: 10.1128/jcm.26.3.504-507.1988.
2
A rapid microneutralization assay for the measurement of neutralizing antibody reactive with human cytomegalovirus.一种用于检测与人巨细胞病毒反应的中和抗体的快速微量中和试验。
J Virol Methods. 1989 Feb;23(2):157-67. doi: 10.1016/0166-0934(89)90129-8.
3
Rapid (24h) neutralization assay for the detection of antibodies to human cytomegalovirus using a monoclonal antibody to an HCMV early nuclear protein.使用针对人巨细胞病毒早期核蛋白的单克隆抗体进行快速（24小时）中和试验以检测人巨细胞病毒抗体。
J Virol Methods. 1988 Oct;22(1):31-40. doi: 10.1016/0166-0934(88)90085-7.
4
Rapid neutralization assay for human cytomegalovirus antibody.人巨细胞病毒抗体快速中和试验
J Clin Microbiol. 1985 Jun;21(6):969-71. doi: 10.1128/jcm.21.6.969-971.1985.
5
Assessment of the presence of cytomegalovirus-neutralizing antibody by a plaque-reduction assay.通过蚀斑减少试验评估巨细胞病毒中和抗体的存在情况。
Rev Infect Dis. 1986 Jul-Aug;8 Suppl 4:S434-8. doi: 10.1093/clinids/8.supplement_4.s434.
6
A microneutralization enzyme immunoassay for antibody to human cytomegalovirus.一种用于检测人巨细胞病毒抗体的微中和酶免疫测定法。
J Immunol Methods. 1993 Feb 26;159(1-2):71-9. doi: 10.1016/0022-1759(93)90143-u.
7
Rapid detection of antibodies against cytomegalovirus induced immediate early and early antigens by an enzyme linked immunosorbent assay.通过酶联免疫吸附试验快速检测抗巨细胞病毒诱导的即刻早期和早期抗原的抗体。
J Clin Pathol. 1984 Feb;37(2):122-5. doi: 10.1136/jcp.37.2.122.
8
Three-day assay for human cytomegalovirus applicable to serum neutralization tests.适用于血清中和试验的人巨细胞病毒三日检测法。
Appl Microbiol. 1973 Jan;25(1):37-9. doi: 10.1128/am.25.1.37-39.1973.
9
Rapid quantitative assay of cytomegalovirus infectivity.巨细胞病毒感染性的快速定量测定
J Virol Methods. 1988 Aug;20(4):333-40. doi: 10.1016/0166-0934(88)90136-x.
10
Rapid detection of cytomegalovirus by tissue culture, centrifugation, and immunofluorescence with a monoclonal antibody to an early nuclear antigen.通过组织培养、离心以及使用针对早期核抗原的单克隆抗体进行免疫荧光检测来快速检测巨细胞病毒。
J Virol Methods. 1987 Jul;16(4):327-38. doi: 10.1016/0166-0934(87)90018-8.
引用本文的文献
1
In-depth summary over cytomegalovirus infection in allogeneic hematopoietic stem cell transplantation recipients.异基因造血干细胞移植受者巨细胞病毒感染的深入总结
Virusdisease. 2021 Sep;32(3):422-434. doi: 10.1007/s13337-021-00728-w. Epub 2021 Jul 28.
2
Human Cytomegalovirus Productively Replicates in Undifferentiated Oral Epithelial Cells.人巨细胞病毒在未分化的口腔上皮细胞中有效复制。
J Virol. 2018 Jul 31;92(16). doi: 10.1128/JVI.00903-18. Print 2018 Aug 15.
3
HCMV Displays a Unique Transcriptome of Immunomodulatory Genes in Primary Monocyte-Derived Cell Types.人巨细胞病毒在原代单核细胞衍生细胞类型中展现出免疫调节基因的独特转录组。
PLoS One. 2016 Oct 19;11(10):e0164843. doi: 10.1371/journal.pone.0164843. eCollection 2016.
4
Human Cytomegalovirus Can Procure Deoxyribonucleotides for Viral DNA Replication in the Absence of Retinoblastoma Protein Phosphorylation.人类巨细胞病毒可在不存在视网膜母细胞瘤蛋白磷酸化的情况下获取脱氧核糖核苷酸用于病毒DNA复制。
J Virol. 2016 Sep 12;90(19):8634-43. doi: 10.1128/JVI.00731-16. Print 2016 Oct 1.
5
Human cytomegalovirus miR-UL112-1 promotes the down-regulation of viral immediate early-gene expression during latency to prevent T-cell recognition of latently infected cells.人类巨细胞病毒miR-UL112-1在潜伏期间促进病毒立即早期基因表达的下调，以防止T细胞识别潜伏感染的细胞。
J Gen Virol. 2016 Sep;97(9):2387-2398. doi: 10.1099/jgv.0.000546. Epub 2016 Jul 13.
6
Perivascular stromal cells as a potential reservoir of human cytomegalovirus.血管周基质细胞作为人类巨细胞病毒的潜在储库。
Am J Transplant. 2014 Apr;14(4):820-30. doi: 10.1111/ajt.12642. Epub 2014 Mar 4.
7
Overview of the diagnosis of cytomegalovirus infection.巨细胞病毒感染的诊断概述。
Infect Disord Drug Targets. 2011 Oct;11(5):466-74. doi: 10.2174/187152611797636703.
8
Human cytomegalovirus is protected from inactivation by reversible binding to villous trophoblasts.人类巨细胞病毒通过与绒毛滋养层细胞可逆结合而免受失活。
Biol Reprod. 2011 Jul;85(1):198-207. doi: 10.1095/biolreprod.110.088567. Epub 2011 Mar 2.
9
Novel microneutralization assay for HCMV using automated data collection and analysis.使用自动数据收集和分析的新型人巨细胞病毒微中和试验。
J Immunol Methods. 2007 Apr 30;322(1-2):82-93. doi: 10.1016/j.jim.2007.02.001. Epub 2007 Feb 23.
10
Novel real-time monitoring system for human cytomegalovirus-infected cells in vitro that uses a green fluorescent protein-PML-expressing cell line.一种用于体外人巨细胞病毒感染细胞的新型实时监测系统，该系统使用表达绿色荧光蛋白-PML的细胞系。
Antimicrob Agents Chemother. 2006 Aug;50(8):2806-13. doi: 10.1128/AAC.01641-05.
本文引用的文献
1
A PLAQUE REDUCTION NEUTRALIZATION TEST FOR HUMAN CYTOMEGALOVIRUS.人巨细胞病毒蚀斑减少中和试验
Proc Soc Exp Biol Med. 1964 Oct;117:145-50. doi: 10.3181/00379727-117-29520.
2
Neutralizing antibodies detect a disulfide-linked glycoprotein complex within the envelope of human cytomegalovirus.中和抗体可检测到人巨细胞病毒包膜内一种二硫键连接的糖蛋白复合物。
Virology. 1984 Jun;135(2):369-78. doi: 10.1016/0042-6822(84)90193-4.
3
Large-scale serological screening for cytomegalovirus antibodies in homosexual males by enzyme-linked immunosorbent assay.采用酶联免疫吸附测定法对男性同性恋者进行巨细胞病毒抗体的大规模血清学筛查。
J Clin Microbiol. 1984 Feb;19(2):200-3. doi: 10.1128/jcm.19.2.200-203.1984.
4
Prevention of cytomegalovirus infection by cytomegalovirus immune globulin after marrow transplantation.骨髓移植后应用巨细胞病毒免疫球蛋白预防巨细胞病毒感染。
Ann Intern Med. 1983 Apr;98(4):442-6. doi: 10.7326/0003-4819-98-4-442.
5
Rapid detection and quantitation of human cytomegalovirus in urine through DNA hybridization.通过DNA杂交技术对尿液中的人巨细胞病毒进行快速检测和定量分析。
N Engl J Med. 1983 Apr 21;308(16):921-5. doi: 10.1056/NEJM198304213081603.
6
Murine monoclonal antibody to a single protein neutralizes the infectivity of human cytomegalovirus.针对单一蛋白质的鼠单克隆抗体可中和人巨细胞病毒的感染性。
Proc Natl Acad Sci U S A. 1984 Feb;81(3):876-80. doi: 10.1073/pnas.81.3.876.
7
Rapid diagnosis of cytomegalovirus infection in immunocompromised patients by detection of early antigen fluorescent foci.通过检测早期抗原荧光病灶快速诊断免疫功能低下患者的巨细胞病毒感染。
Lancet. 1984 Dec 1;2(8414):1242-5. doi: 10.1016/s0140-6736(84)92797-1.
8
Detection of cytomegaloviremia using monoclonal antibodies.使用单克隆抗体检测巨细胞病毒血症
Dev Biol Stand. 1984;57:287-91.
9
Cytomegalovirus infects human lymphocytes and monocytes: virus expression is restricted to immediate-early gene products.巨细胞病毒感染人类淋巴细胞和单核细胞：病毒表达仅限于立即早期基因产物。
Proc Natl Acad Sci U S A. 1984 Oct;81(19):6134-8. doi: 10.1073/pnas.81.19.6134.
10
Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen.通过低速离心和使用针对早期抗原的单克隆抗体，快速检测接种尿液标本的MRC-5细胞中的巨细胞病毒。
J Clin Microbiol. 1984 Jun;19(6):917-9. doi: 10.1128/jcm.19.6.917-919.1984.
Zotero 插件