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A PLAQUE REDUCTION NEUTRALIZATION TEST FOR HUMAN CYTOMEGALOVIRUS.人巨细胞病毒蚀斑减少中和试验
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Neutralizing antibodies detect a disulfide-linked glycoprotein complex within the envelope of human cytomegalovirus.中和抗体可检测到人巨细胞病毒包膜内一种二硫键连接的糖蛋白复合物。
Virology. 1984 Jun;135(2):369-78. doi: 10.1016/0042-6822(84)90193-4.
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Large-scale serological screening for cytomegalovirus antibodies in homosexual males by enzyme-linked immunosorbent assay.采用酶联免疫吸附测定法对男性同性恋者进行巨细胞病毒抗体的大规模血清学筛查。
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Prevention of cytomegalovirus infection by cytomegalovirus immune globulin after marrow transplantation.骨髓移植后应用巨细胞病毒免疫球蛋白预防巨细胞病毒感染。
Ann Intern Med. 1983 Apr;98(4):442-6. doi: 10.7326/0003-4819-98-4-442.
5
Rapid detection and quantitation of human cytomegalovirus in urine through DNA hybridization.通过DNA杂交技术对尿液中的人巨细胞病毒进行快速检测和定量分析。
N Engl J Med. 1983 Apr 21;308(16):921-5. doi: 10.1056/NEJM198304213081603.
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Murine monoclonal antibody to a single protein neutralizes the infectivity of human cytomegalovirus.针对单一蛋白质的鼠单克隆抗体可中和人巨细胞病毒的感染性。
Proc Natl Acad Sci U S A. 1984 Feb;81(3):876-80. doi: 10.1073/pnas.81.3.876.
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Rapid diagnosis of cytomegalovirus infection in immunocompromised patients by detection of early antigen fluorescent foci.通过检测早期抗原荧光病灶快速诊断免疫功能低下患者的巨细胞病毒感染。
Lancet. 1984 Dec 1;2(8414):1242-5. doi: 10.1016/s0140-6736(84)92797-1.
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Detection of cytomegaloviremia using monoclonal antibodies.使用单克隆抗体检测巨细胞病毒血症
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Cytomegalovirus infects human lymphocytes and monocytes: virus expression is restricted to immediate-early gene products.巨细胞病毒感染人类淋巴细胞和单核细胞：病毒表达仅限于立即早期基因产物。
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10
Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen.通过低速离心和使用针对早期抗原的单克隆抗体，快速检测接种尿液标本的MRC-5细胞中的巨细胞病毒。
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利用针对病毒主要立即早期蛋白的单克隆抗体对巨细胞病毒进行快速定量及中和抗体检测。

Rapid quantitation of cytomegalovirus and assay of neutralizing antibody by using monoclonal antibody to the major immediate-early viral protein.

作者信息

Chou S W, Scott K M

机构信息

Medical Service, Veterans Administration Medical Center, Portland, Oregon.

出版信息

J Clin Microbiol. 1988 Mar;26(3):504-7. doi: 10.1128/jcm.26.3.504-507.1988.

DOI:10.1128/jcm.26.3.504-507.1988
PMID:2833529
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC266321/
Abstract

An overnight assay, based on staining cytomegalovirus-infected cells with monoclonal antibody to the 72,000-molecular-weight major immediate-early viral protein, was compared with a conventional 14-day plaque assay for quantitation of cell-free stocks of cytomegalovirus laboratory strain AD-169 and 20 other clinical strains. Viral titers were quantitatively similar when determined by either method, but centrifugation of monolayers during inoculation enhanced viral infectivity an average of 4.1-fold. When used for scoring neutralizing antibody assays, monoclonal antibody staining yielded titers within one dilution of 14-day plaque-reduction assays in 54 of 56 titrations. Of 21 cytomegalovirus strains, 2 were not recognized by the monoclonal antibody used. Assay with monoclonal antibody offers a rapid and accurate alternative to plaque assays for quantitation or neutralization of cytomegalovirus.

摘要

基于用针对分子量为72,000的主要立即早期病毒蛋白的单克隆抗体对巨细胞病毒感染细胞进行染色的过夜检测法，与用于定量巨细胞病毒实验室毒株AD - 169和其他20种临床毒株的无细胞毒株的传统14天蚀斑检测法进行了比较。通过任一种方法测定时，病毒滴度在数量上相似，但接种期间单层细胞的离心平均使病毒感染性提高了4.1倍。当用于中和抗体检测的评分时，在56次滴定中的54次中，单克隆抗体染色产生的滴度与14天蚀斑减少检测法的滴度相差不超过一个稀释度。在21种巨细胞病毒毒株中，有2种未被所用的单克隆抗体识别。用单克隆抗体进行检测为巨细胞病毒的定量或中和提供了一种快速且准确的替代蚀斑检测法的方法。