Phoophitphong D, Srisuwatanasagul S, Tummaruk P
Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok, 10900, Thailand.
Anat Histol Embryol. 2017 Aug;46(4):334-341. doi: 10.1111/ahe.12274. Epub 2017 May 2.
This study aimed to investigate leptin immuno-staining of the porcine ovary in different reproductive stages. Ovaries from 21 gilts were collected from slaughterhouses. The ovarian tissue sections were incubated with a polyclonal anti-leptin as a primary antibody. The immuno-staining in ovarian tissue compartments was calculated using imaging software. Leptin immuno-staining was found in primordial, primary, preantral and antral follicles. Leptin immuno-staining was expressed in the oocyte and granulosa and theca interna layers in both preantral and antral follicles. In the corpora lutea, leptin immuno-staining was found in the cytoplasm of the luteal cells. The leptin immuno-staining in the granulosa cell layer of preantral follicles did not differ compared to antral follicles (90.7 and 91.3%, respectively, P > 0.05). However, the leptin immuno-staining in the theca interna layer of preantral follicles was lower than antral follicles (49.4 and 74.3%, respectively, P < 0.001). There was no difference in leptin immuno-staining in the granulosa cell layer between follicular and luteal phases (92.4 and 89.7%, respectively, P > 0.05). However, the leptin immuno-staining in the theca interna layer of follicular phase was greater than that in the luteal phase (72.7 and 51.0%, respectively, P < 0.001). These findings indicated that leptin exists in different compartments of the porcine ovary, including the oocyte, granulosa cells, theca interna cells, corpus luteum, blood vessel and smooth muscles. Therefore, this morphological study confirmed a close relationship between leptin and ovarian function in the pig.
本研究旨在调查不同生殖阶段猪卵巢的瘦素免疫染色情况。从屠宰场收集21头后备母猪的卵巢。卵巢组织切片用多克隆抗瘦素作为一抗进行孵育。使用成像软件计算卵巢组织各部分的免疫染色。在原始卵泡、初级卵泡、窦前卵泡和窦卵泡中均发现瘦素免疫染色。在窦前卵泡和窦卵泡的卵母细胞、颗粒细胞和卵泡内膜层中均有瘦素免疫染色表达。在黄体中，瘦素免疫染色存在于黄体细胞的细胞质中。窦前卵泡颗粒细胞层的瘦素免疫染色与窦卵泡相比无差异（分别为90.7%和91.3%，P>0.05）。然而，窦前卵泡卵泡内膜层的瘦素免疫染色低于窦卵泡（分别为49.4%和74.3%，P<0.001）。卵泡期和黄体期颗粒细胞层的瘦素免疫染色无差异（分别为92.4%和89.7%，P>0.05）。然而，卵泡期卵泡内膜层的瘦素免疫染色大于黄体期（分别为72.7%和51.0%，P<0.001）。这些发现表明瘦素存在于猪卵巢的不同部分，包括卵母细胞、颗粒细胞、卵泡内膜细胞、黄体、血管和平滑肌。因此，这项形态学研究证实了瘦素与猪卵巢功能之间的密切关系。
