Kim Jun-Hwan, Oh Chul Woong, Kang Ju-Chan
a West Sea Fisheries Research Institute, National Institute of Fisheries Science , Incheon 22383 , South Korea.
b Department of Marine Biology , Pukyong National University , Busan 48513 , South Korea.
J Aquat Anim Health. 2017 Jun;29(2):112-119. doi: 10.1080/08997659.2017.1307286.
This study was conducted to assess toxic effects of dietary lead (Pb) exposure on Korean Rockfish Sebastes schlegelii. Juvenile rockfish were used to evaluate the oxidative stress, neurotoxicity, and metallothionein (MT) gene expression after dietary exposure to lead (as Pb; 0, 30, 60, 120 and 240 mg/kg). Superoxide dismutase (SOD) activity, a measure of oxidative stress, was substantially elevated in the livers and gills of fish given dietary Pb greater than 60 mg/kg. Glutathione S-transferase (GST) activity in the liver and gill was significantly increased by dietary Pb > 60 mg/kg. A significant decrease in glutathione (GSH) level was observed in fish liver after exposure to dietary Pb > 30 mg/kg and in the gill after treatment with dietary Pb > 120 mg/kg. Acecyltholinesterase (AChE) was noticeably decreased in the brain by dietary Pb > 120 mg/kg and in the muscle by dietary Pb > 60 mg/kg. Metallothionein gene expression in the liver was stimulated significantly by the Pb exposure. Because dietary Pb exposure had a toxic effect on antioxidant responses, a neurotransmitter, and a specific immune expression in rockfish, the results of this study can be used to determine potential useful markers of Pb toxicity. Received June 11, 2016; accepted March 10, 2017.
本研究旨在评估膳食铅（Pb）暴露对韩国岩鱼许氏平鲉的毒性作用。利用幼鱼评估膳食暴露于铅（以Pb计；0、30、60、120和240mg/kg）后的氧化应激、神经毒性和金属硫蛋白（MT）基因表达。超氧化物歧化酶（SOD）活性是氧化应激的一个指标，在膳食铅含量大于60mg/kg的鱼的肝脏和鳃中显著升高。膳食铅含量大于60mg/kg时，肝脏和鳃中的谷胱甘肽S-转移酶（GST）活性显著增加。在膳食铅含量大于30mg/kg的鱼肝中以及膳食铅含量大于120mg/kg的鳃中观察到谷胱甘肽（GSH）水平显著降低。膳食铅含量大于120mg/kg时，脑中乙酰胆碱酯酶（AChE）明显降低，膳食铅含量大于60mg/kg时，肌肉中AChE明显降低。铅暴露显著刺激了肝脏中金属硫蛋白基因的表达。由于膳食铅暴露对岩鱼的抗氧化反应、神经递质和特定免疫表达产生了毒性作用，本研究结果可用于确定铅毒性的潜在有用标志物。2016年6月11日收到；2017年3月10日接受。
