Department of Aquatic Life Medicine, Pukyong National University, Busan, 608-737, Republic of Korea.
Department of Aquatic Life Medicine, Pukyong National University, Busan, 608-737, Republic of Korea.
Chemosphere. 2017 Mar;171:520-527. doi: 10.1016/j.chemosphere.2016.12.094. Epub 2016 Dec 21.
Juvenile rockfish Sebastes schlegelii were exposed to varying levels of dietary lead (Pb) at 0, 120 and 240 mg/L, and ascorbic acid (AsA) at 100, 200 and 400 mg/L for four weeks. Antioxidant responses such as superoxide dismutase (SOD), glutathione S-transferase (GST), and glutathione (GSH) were analyzed to assess oxidative stress. SOD and GST activity in the liver and gills were considerably elevated by dietary Pb. In contrast, GSH levels in the liver and gills were significantly reduced following Pb exposure. High levels of AsA supplementation attenuated the increase in SOD and GST activity and reduction in GSH levels. The metallothionein gene (MT) in the liver was notably stimulated by Pb exposure, and AsA supplementation attenuated this increase. With respect to neurotoxicity, acetylcholinesterase (AChE) activity was substantially inhibited in the brain and muscle following Pb exposure. AsA supplementation also attenuated AChE inhibition following Pb exposure. The results of this study presented Pb exposure affected rockfish as toxicity, and AsA was effective to alleviate toxic effects of Pb.
将幼年岩鱼（Sebastes schlegelii）暴露于 0、120 和 240mg/L 的不同水平的膳食铅（Pb）和 100、200 和 400mg/L 的抗坏血酸（AsA）中 4 周。分析抗氧化反应，如超氧化物歧化酶（SOD）、谷胱甘肽 S-转移酶（GST）和谷胱甘肽（GSH），以评估氧化应激。肝脏和鳃中的 SOD 和 GST 活性因膳食 Pb 而显著升高。相比之下，Pb 暴露后肝脏和鳃中的 GSH 水平明显降低。高水平的 AsA 补充可减弱 SOD 和 GST 活性的增加以及 GSH 水平的降低。肝脏中的金属硫蛋白基因（MT）因 Pb 暴露而明显受到刺激，而 AsA 补充可减弱这种增加。就神经毒性而言，Pb 暴露后大脑和肌肉中的乙酰胆碱酯酶（AChE）活性显著受到抑制。AsA 补充也可减轻 Pb 暴露后 AChE 的抑制。本研究的结果表明，Pb 暴露对岩鱼有毒性，而 AsA 可有效减轻 Pb 的毒性作用。
