Sun Chong-Ling, Wei Jun, Bi Li-Qi
Department of Rheumatology and Immunology, The First College of Clinical Medical Science, China Three Gorges University, Yichang, and Department of Rheumatology and Immunology, China-Japan Union Hospital of Jilin University, Changchun, China.
Pharmacology. 2017;100(1-2):40-49. doi: 10.1159/000451027. Epub 2017 May 4.
The present study was intended to elucidate the effect of rutin, a flavonoid, on arthritis in complete Freund's adjuvant-induced arthritic rats. The present study showed that more pronounced effect has been observed in the case of 15 mg/kg dose of rutin, with significant reduction in paw diameter together with positive modulation of hematological parameters as compared to 2 other tested doses. A significant upsurge in the level of superoxide dismutase, glutathione peroxidase and glutathione were observed together with decrease in the level of malondialdehyde after treatment with rutin in a dose-dependent manner. Furthermore, the effect of rutin on the tumor necrosis factor-α and interleukin-1β in arthritic rats showed does-dependent lowering of these cytokines with maximum benefit at 15 mg/kg dose and the level of both NF-κB p65 and NF-κBp65 (Ser536) has been significantly reduced in the presence of rutin. Histopathological examination showed that the inflammatory cells infiltration, synovial hyperplasia, pannus formation and cartilage and bone erosion had considerably improved on administration of rutin. In conclusion, our paper strongly demonstrated the protective effect of rutin against the rheumatoid arthritis involved via suppression of NF-κB p65 protein expression.
本研究旨在阐明类黄酮芦丁对完全弗氏佐剂诱导的关节炎大鼠的关节炎的影响。本研究表明，与其他两个测试剂量相比，15mg/kg剂量的芦丁效果更为显著，爪直径显著减小，血液学参数得到正向调节。用芦丁处理后，超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽水平显著升高，丙二醛水平降低，且呈剂量依赖性。此外，芦丁对关节炎大鼠肿瘤坏死因子-α和白细胞介素-1β的影响显示，这些细胞因子呈剂量依赖性降低，在15mg/kg剂量时获益最大，并且在芦丁存在的情况下，NF-κB p65和NF-κBp65（Ser536）的水平均显著降低。组织病理学检查显示，给予芦丁后，炎症细胞浸润、滑膜增生、血管翳形成以及软骨和骨侵蚀均有明显改善。总之，我们的论文有力地证明了芦丁通过抑制NF-κB p65蛋白表达对类风湿性关节炎具有保护作用。
