Department of Herbal Pharmacology, College of Korean Medicine, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam-si 13120, Republic of Korea.
Naturalis Inc., 6 Daewangpangyo-ro, Bundang-gu, Seongnam-si 13549, Republic of Korea.
Int J Mol Sci. 2024 Sep 4;25(17):9594. doi: 10.3390/ijms25179594.
Osteoarthritis (OA) is an age-related disease characterized by inflammation, pain, articular cartilage damage, synovitis, and irreversible disability. (Thunb.) Makino (GP), a herbal medicine traditionally used in East Asia for its anti-inflammatory properties, was investigated for its potential to modulate OA pathology and symptoms. This study evaluated GP's efficacy in inhibiting pain, functional decline, and cartilage destruction in monosodium iodoacetate-induced OA and acetic acid-induced writhing models. Additionally, the effects of GP on OA-related inflammatory targets were assessed via mRNA and protein expression in rat knee cartilage and lipopolysaccharide-induced RAW 264.7 cells. The GP group demonstrated significant pain relief, functional improvement, and cartilage protection. Notably, GP inhibited key inflammatory mediators, including interleukin (IL)-1β, IL-6, matrix metalloproteinases (MMP)-3 and MMP-13, cyclooxygenase-2, and prostaglandin E receptor 2, surpassing the effects of active controls. These findings suggest that GP is a promising candidate for disease-modifying OA drugs and warrants further comprehensive studies.
骨关节炎（OA）是一种与年龄相关的疾病，其特征为炎症、疼痛、关节软骨损伤、滑膜炎和不可逆转的残疾。（Thunb.）Makino（GP）是一种传统上用于东亚的草药，具有抗炎特性，被研究用于调节 OA 病理和症状的潜力。本研究评估了 GP 在抑制疼痛、功能下降和软骨破坏方面的功效，使用单碘乙酸盐诱导的 OA 和乙酸诱导的扭体模型进行了评估。此外，通过大鼠膝关节软骨和脂多糖诱导的 RAW 264.7 细胞中的 mRNA 和蛋白质表达，评估了 GP 对 OA 相关炎症靶点的影响。GP 组表现出显著的疼痛缓解、功能改善和软骨保护。值得注意的是，GP 抑制了关键的炎症介质，包括白细胞介素（IL）-1β、IL-6、基质金属蛋白酶（MMP）-3 和 MMP-13、环氧化酶-2 和前列腺素 E 受体 2，超过了阳性对照的效果。这些发现表明，GP 是一种有前途的治疗 OA 的药物候选物，值得进一步进行全面研究。
