Yang Li, Liu Ronghua, Fan Aiguo, Zhao Jingjing, Zhang Yong, He Junwei
Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
Department of Pharmacy, Guangdong Women and Children Hospital, Guangzhou, China.
Front Pharmacol. 2020 Dec 11;11:584849. doi: 10.3389/fphar.2020.584849. eCollection 2020.
Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease without effective and beneficial drugs. Many traditional folk medicines have been proven to be effective in treating RA. Among these, the root of Hance has been widely used as a traditional remedy against RA in China, but there is no scientific basis yet. The aim of this study was to investigate for the first time the chemical compositions and therapeutic effect of on adjuvant-induced arthritis (AIA) model in rats. 73 compounds were identified from based on ultra-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-qTOF-MS/MS), and flavonoids may be partly responsible for the major anti-arthritic effect. In parallel, the extract at 160, 320, and 640 mg/kg/day were orally administered to rats for 22 days after post-administration adjuvant. The results showed that remarkably ameliorated histological lesions of the knee joint, increased body weight growth, decreased arthritis score, reduced thymus and spleen indices in model rats. Moreover, treatment persuasively downregulated the levels of rheumatoid factor (RF), C-reactive protein (CRP), tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), IL-6, IL-17, cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LOX) and matrix metalloproteinase-2 (MMP-2), and observably upregulated IL-4 and IL-10 levels in model rats. These findings suggest that has a prominent anti-RA effect on AIA rats by modulating the inflammatory responses, and supports the traditional folk use of this plant.
类风湿性关节炎（RA）是一种尚无有效有益药物的慢性自身免疫性炎症性疾病。许多传统民间药物已被证明对治疗RA有效。其中，汉防己根在中国已被广泛用作治疗RA的传统药物，但尚无科学依据。本研究的目的是首次研究汉防己对大鼠佐剂性关节炎（AIA）模型的化学成分和治疗效果。基于超高效液相色谱-四极杆飞行时间串联质谱（UPLC-qTOF-MS/MS）从汉防己中鉴定出73种化合物，黄酮类化合物可能部分负责主要的抗关节炎作用。同时，在给予佐剂后，将汉防己提取物以160、320和640mg/kg/天的剂量口服给予大鼠22天。结果表明，汉防己显著改善了模型大鼠膝关节的组织学损伤，增加了体重增长，降低了关节炎评分，降低了胸腺和脾脏指数。此外，汉防己治疗有力地下调了模型大鼠中类风湿因子（RF）、C反应蛋白（CRP）、肿瘤坏死因子α（TNF-α）、白细胞介素-1β（IL-1β）、IL-6、IL-17、环氧化酶-2（COX-2）、5-脂氧合酶（5-LOX）和基质金属蛋白酶-2（MMP-2）的水平，并显著上调了IL-4和IL-10水平。这些发现表明，汉防己通过调节炎症反应对AIA大鼠具有显著的抗RA作用，并支持了这种植物的传统民间用途。
