Aziz Mina, Sturtevant Drew, Winston Jordan, Collakova Eva, Jelesko John G, Chapman Kent D
Department of Biological Sciences, BioDiscovery Institute, University of North Texas, Denton, TX 76203, USA.
Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech University, Blacksburg, VA 24061, USA.
Molecules. 2017 Apr 29;22(5):711. doi: 10.3390/molecules22050711.
Urushiols are the allergenic components of (poison ivy) as well as other Toxicodendron species. They are alk-(en)-yl catechol derivatives with a 15- or 17-carbon side chain having different degrees of unsaturation. Although several methods have been developed for analysis of urushiols in plant tissues, the in situ localization of the different urushiol congeners has not been reported. Here, we report on the first analysis of urushiols in poison ivy stems by matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). Our results show that the urushiol congeners with 15-carbon side chains are mainly localized to the resin ducts, while those with 17-carbon side chains are widely distributed in cortex and vascular tissues. The presence of these urushiols in stem extracts of poison ivy seedlings was confirmed by GC-MS. These novel findings provide new insights into the spatial tissue distribution of urushiols that might be biosynthetically or functionally relevant.
漆酚是（毒葛）以及其他漆树属植物的致敏成分。它们是具有15或17个碳原子侧链、不饱和度不同的烷基（烯基）邻苯二酚衍生物。尽管已经开发了几种用于分析植物组织中漆酚的方法，但不同漆酚同系物的原位定位尚未见报道。在此，我们报告了通过基质辅助激光解吸/电离质谱成像（MALDI-MSI）对毒葛茎中漆酚的首次分析。我们的结果表明，具有15个碳原子侧链的漆酚同系物主要定位于树脂道，而具有17个碳原子侧链的漆酚同系物广泛分布于皮层和维管组织中。通过气相色谱-质谱联用（GC-MS）证实了毒葛幼苗茎提取物中存在这些漆酚。这些新发现为漆酚的空间组织分布提供了新的见解，这些分布可能在生物合成或功能上具有相关性。
