Endemic Species Research Institute, Nantou 552, Taiwan.
Int J Mol Sci. 2013 Oct 14;14(10):20414-26. doi: 10.3390/ijms141020414.
Poison ivy, Toxicodendron radicans, and poison oaks, T. diversilobum and T. pubescens, are perennial woody species of the Anacardiaceae and are poisonous, containing strong allergens named urushiols that cause allergic contact dermatitis. Poison ivy is a species distributed from North America to East Asia, while T. diversilobum and T. pubescens are distributed in western and eastern North America, respectively. Phylogreography and population structure of these species remain unclear. Here, we developed microsatellite markers, via constructing a magnetic enriched microsatellite library, from poison ivy. We designed 51 primer pairs, 42 of which successfully yielded products that were subsequently tested for polymorphism in poison oak, and three subspecies of poison ivy. Among the 42 loci, 38 are polymorphic, while 4 are monomorphic. The number of alleles and the expected heterozygosity ranged from 1 to 12 and from 0.10 to 0.87, respectively, in poison ivy, while varied from 2 to 8 and, from 0.26 to 0.83, respectively in poison oak. Genetic analysis revealed distinct differentiation between poison ivy and poison oak, whereas slight genetic differentiation was detected among three subspecies of poison ivy. These highly polymorphic microsatellite fingerprints enable biologists to explore the population genetics, phylogeography, and speciation in Toxicodendron.
毒葛、毒栎，漆树科的多年生木本植物，是有毒的，含有强烈的过敏原漆酚，会引起过敏性接触性皮炎。毒葛分布于从北美洲到东亚，而毒栎和毒漆分布于北美洲的西部和东部。这些物种的系统地理学和种群结构仍不清楚。在这里，我们通过构建磁富集微卫星文库，从毒葛中开发了微卫星标记。我们设计了 51 对引物，其中 42 对成功产生了产物，随后在毒栎和毒葛的三个亚种中进行了多态性测试。在 42 个位点中，38 个是多态的，4 个是单态的。毒葛的等位基因数和期望杂合度范围分别为 1 到 12 和 0.10 到 0.87，而毒栎的范围分别为 2 到 8 和 0.26 到 0.83。遗传分析表明，毒葛和毒栎之间存在明显的分化，而毒葛的三个亚种之间存在轻微的遗传分化。这些高度多态的微卫星指纹图谱使生物学家能够探索漆树属的种群遗传学、系统地理学和物种形成。
