Weisberg Alexandra J, Kim Gunjune, Westwood James H, Jelesko John G
Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97330, USA.
Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA 24061, USA.
Genes (Basel). 2017 Nov 10;8(11):317. doi: 10.3390/genes8110317.
Contact with poison ivy plants is widely dreaded because they produce a natural product called urushiol that is responsible for allergenic contact delayed-dermatitis symptoms lasting for weeks. For this reason, the catchphrase most associated with poison ivy is "leaves of three, let it be", which serves the purpose of both identification and an appeal for avoidance. Ironically, despite this notoriety, there is a dearth of specific knowledge about nearly all other aspects of poison ivy physiology and ecology. As a means of gaining a more molecular-oriented understanding of poison ivy physiology and ecology, Next Generation DNA sequencing technology was used to develop poison ivy root and leaf RNA-seq transcriptome resources. De novo assembled transcriptomes were analyzed to generate a core set of high quality expressed transcripts present in poison ivy tissue. The predicted protein sequences were evaluated for similarity to SwissProt homologs and InterProScan domains, as well as assigned both GO terms and KEGG annotations. Over 23,000 simple sequence repeats were identified in the transcriptome, and corresponding oligo nucleotide primer pairs were designed. A pan-transcriptome analysis of existing Anacardiaceae transcriptomes revealed conserved and unique transcripts among these species.
接触毒葛植物令人闻风丧胆，因为它们会产生一种名为漆酚的天然产物，这种物质会导致过敏性接触延迟性皮炎症状，持续数周。因此，与毒葛最相关的口头禅是“三叶要留意”，它既有助于识别毒葛，也呼吁人们避开它。具有讽刺意味的是，尽管毒葛声名狼藉，但人们对其生理和生态几乎所有其他方面的具体知识却知之甚少。作为一种从分子层面更深入了解毒葛生理和生态的方法，研究人员利用新一代DNA测序技术开发了毒葛根和叶的RNA测序转录组资源。对从头组装的转录组进行分析，以生成毒葛组织中存在的一组高质量核心表达转录本。对预测的蛋白质序列进行评估，以确定其与瑞士蛋白质数据库（SwissProt）同源物和InterProScan结构域的相似性，并赋予其基因本体（GO）术语和京都基因与基因组百科全书（KEGG）注释。在转录组中鉴定出超过23000个简单序列重复，并设计了相应的寡核苷酸引物对。对漆树科现有转录组进行的泛转录组分析揭示了这些物种中保守和独特的转录本。
