Winarto Hariyono, Tan Marselina Irasonia, Sadikin Mohamad, Wanandi Septelia Inawati
Gynecologic Oncology Division, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Doctoral Biomedical Science Program, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Transl Oncogenomics. 2017 Feb 24;9:1177272716689818. doi: 10.1177/1177272716689818. eCollection 2017.
Oxidative stress is considered an important factor in the development of endometriosis, including its malignant transformation. Previous studies have found that AT-rich interactive domain 1A (), a tumor suppressor gene, is frequently mutated and inactivated in endometriosis-associated ovarian cancer (EAOC), and such a change in this gene is considered an early event in malignant transformation. We observed oxidative stress status by measuring the activity of the antioxidant enzyme manganese superoxide dismutase (MnSOD), malondialdehyde (MDA), and gene expression in tissue samples from patients with endometriosis, EAOC, or non-endometriosis-associated ovarian cancer (non-EAOC). We also induced oxidative stress in the cultured cells from patients with primary endometriosis by adding HO and tested for any alteration of gene expression based on different HO concentrations. The results showed that MnSOD activity in endometriosis and EAOC was lower than in non-EAOC, but MDA levels were higher. This study also showed that oxidative stress reduced gene expression.
氧化应激被认为是子宫内膜异位症发生发展的一个重要因素，包括其恶性转化。先前的研究发现，富含AT的交互结构域1A（），一种肿瘤抑制基因，在子宫内膜异位症相关卵巢癌（EAOC）中经常发生突变并失活，并且该基因的这种变化被认为是恶性转化的早期事件。我们通过测量抗氧化酶锰超氧化物歧化酶（MnSOD）的活性、丙二醛（MDA）以及子宫内膜异位症患者、EAOC患者或非子宫内膜异位症相关卵巢癌（非EAOC）患者组织样本中的基因表达来观察氧化应激状态。我们还通过添加HO在原发性子宫内膜异位症患者的培养细胞中诱导氧化应激，并根据不同的HO浓度测试基因表达的任何变化。结果表明，子宫内膜异位症和EAOC中的MnSOD活性低于非EAOC，但MDA水平较高。这项研究还表明，氧化应激降低了基因表达。
