Poggio Claudio, Riva Paolo, Chiesa Marco, Colombo Marco, Pietrocola Giampiero
Department of Clinical-Surgical, Diagnostic and Pediatric Sciences - Section of Dentistry, University of Pavia, Pavia, Italy.
Departement of Molecular Medicine, Unit of Biochemistry, University of Pavia, Pavia, Italy.
J Clin Exp Dent. 2017 Apr 1;9(4):e574-e578. doi: 10.4317/jced.53724. eCollection 2017 Apr.
The aim of the present study is to evaluate and compare the cytotoxic effects of eight root canal sealers (BioRoot RCS, TotalFill BC Sealer, MTA Fillapex, Sealapex, AH Plus, EasySeal, Pulp Canal Sealer, N2) on immortalized human gingival fibroblasts over a period of 24, 48 and 72 hours.
Immortalized human gingival fibroblast-1 HGF-1 (ATCC CRL-2014) were incubated. Root canal sealers were then placed into sterile, cylindrical Teflon moulds. The extraction was made eluting the sealers in cell culture medium. Cells (1 × 104) were seeded in each well of a 96-well plate and incubated for 24 h at 37°C. Cultures were then exposed to 100 μL of the extracts medium. The percentage of viable cells in each well was calculated relative to control cells set to 100%.
BioRoot RCS and TotalFill BC Sealer extracted for 24h showed no cytotoxic effect, while it was mild by using 48 and 72 h extracts. No cytotoxic effect was measured by using AH Plus medium eluted for 24 h, while it was moderate after 48 h and severe after 72 h. Pulp Canal Sealer, Sealapex and N2 showed moderately cytotoxic activity for all the extraction times. EasySeal and MTA Fillapex remained severely or borderline mildly cytotoxic for all the extraction times.
In the present study only BioRoot RCS, TotalFill BC Sealer and AH Plus showed no cytotoxic effects at least in the first 24h. All the other sealers revealed moderately or severely cytotoxic activity during all the extraction times. Cytotoxicity, gingival fibroblast, MTT test, root canal sealer.
本研究的目的是评估和比较八种根管封闭剂（BioRoot RCS、TotalFill BC封闭剂、MTA Fillapex、Sealapex、AH Plus、EasySeal、牙髓根管封闭剂、N2）在24、48和72小时内对永生化人牙龈成纤维细胞的细胞毒性作用。
培养永生化人牙龈成纤维细胞-1 HGF-1（ATCC CRL-2014）。然后将根管封闭剂放入无菌圆柱形聚四氟乙烯模具中。通过在细胞培养基中洗脱封闭剂进行提取。将细胞（1×10⁴）接种到96孔板的每个孔中，并在37°C下孵育24小时。然后将培养物暴露于100μL提取培养基中。计算每个孔中活细胞的百分比相对于设定为100%的对照细胞的比例。
提取24小时的BioRoot RCS和TotalFill BC封闭剂未显示细胞毒性作用，而使用48小时和72小时提取物时细胞毒性作用较轻。使用洗脱24小时的AH Plus培养基未检测到细胞毒性作用，而48小时后为中度，72小时后为重度。牙髓根管封闭剂、Sealapex和N2在所有提取时间均显示出中度细胞毒性活性。EasySeal和MTA Fillapex在所有提取时间均保持重度或临界轻度细胞毒性。
在本研究中，仅BioRoot RCS、TotalFill BC封闭剂和AH Plus至少在最初24小时内未显示细胞毒性作用。所有其他封闭剂在所有提取时间均显示出中度或重度细胞毒性活性。细胞毒性、牙龈成纤维细胞、MTT试验、根管封闭剂。
