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与传统树脂基根管封闭剂相比，生物陶瓷根管封闭剂的细胞毒性和遗传毒性

Cytotoxicity and genotoxicity of bioceramic root canal sealers compared to conventional resin-based sealer.

作者信息

Radwanski Mateusz, Rozpedek-Kaminska Wioletta, Galita Grzegorz, Siwecka Natalia, Sokolowski Jerzy, Majsterek Ireneusz, Özcan Mutlu, Lukomska-Szymanska Monika

机构信息

Department of Endodontics, Medical University of Lodz, Lodz, Poland.

Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, Lodz, Poland.

出版信息

Sci Rep. 2024 Feb 19;14(1):4124. doi: 10.1038/s41598-024-54726-1.

DOI:10.1038/s41598-024-54726-1
PMID:38374199
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10876547/
Abstract

The aim of this study was to evaluate cytotoxicity and genotoxicity of calcium-silicate based sealers and comparing them with a gold standard-an epoxy-based sealant. Two experimental cell lines were used, gingival fibroblasts (hGF) and monocyte/macrophage peripheral blood cell line (SC). The cytotoxicity (XTT assay) and genotoxicity (comet assay) were evaluated both after 24-h and 48-h incubation. Additionally, after 48-h incubation, the cell apoptosis and cell cycle progression was detected. BioRoot Flow induced a significant decrease in hGF cells viability compared to the negative control groups both after 24-h (p < 0.001) and 48-h incubation (p < 0.01). In group with SC cells, after 24-h incubation significant increase in cells viability was detected for AH Plus Bioceramic Sealer in comparison to negative control (p < 0.05). BioRoot Flow and BioRoot RCS can be considered potentially genotoxic for the hGF cells after 48-h incubation (> 20% DNA damage). BioRoot Flow and BioRoot RCS, may have potential genotoxic effects and induce apoptosis in hGF cells which may irritate periapical tissues, resulting in a delayed healing. The findings of the study would be useful in selection of an appropriate sealant for root canal filling without causing cytotoxicity and genotoxicity.

摘要

本研究的目的是评估硅酸钙类封闭剂的细胞毒性和遗传毒性，并将其与金标准——环氧类封闭剂进行比较。使用了两种实验细胞系，牙龈成纤维细胞（hGF）和单核细胞/巨噬细胞外周血细胞系（SC）。在孵育24小时和48小时后，分别评估细胞毒性（XTT法）和遗传毒性（彗星试验）。此外，在孵育48小时后，检测细胞凋亡和细胞周期进程。与阴性对照组相比，BioRoot Flow在孵育24小时（p < 0.001）和48小时（p < 0.01）后均导致hGF细胞活力显著降低。在SC细胞组中，孵育24小时后，与阴性对照相比，AH Plus生物陶瓷封闭剂的细胞活力显著增加（p < 0.05）。孵育48小时后，BioRoot Flow和BioRoot RCS对hGF细胞可被认为具有潜在遗传毒性（> 20% DNA损伤）。BioRoot Flow和BioRoot RCS可能具有潜在的遗传毒性作用，并诱导hGF细胞凋亡，这可能会刺激根尖周组织，导致愈合延迟。该研究结果将有助于选择合适的根管充填封闭剂，而不会引起细胞毒性和遗传毒性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/10876547/05306868d30f/41598_2024_54726_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/10876547/0fa3dacabe28/41598_2024_54726_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/10876547/1484f7d0d8a5/41598_2024_54726_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/10876547/463f11ac23a0/41598_2024_54726_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/10876547/f4abb39454ec/41598_2024_54726_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/10876547/8c991638159c/41598_2024_54726_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/10876547/63c77f6e007b/41598_2024_54726_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/10876547/5af96f3bb310/41598_2024_54726_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/10876547/05306868d30f/41598_2024_54726_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/10876547/0fa3dacabe28/41598_2024_54726_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/10876547/1484f7d0d8a5/41598_2024_54726_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/10876547/463f11ac23a0/41598_2024_54726_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/10876547/f4abb39454ec/41598_2024_54726_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/10876547/8c991638159c/41598_2024_54726_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/10876547/63c77f6e007b/41598_2024_54726_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/10876547/5af96f3bb310/41598_2024_54726_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/10876547/05306868d30f/41598_2024_54726_Fig8_HTML.jpg

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