Almeida Marcela-Milanezi, Rodrigues Clarissa-Teles, Matos Adriana-Arruda, Carvalho Kleber-Kildare-Teodoro, Silva Emmanuel-João-Nogueira-Leal, Duarte Marco-Antonio-Hungaro, Oliveira Rodrigo-Cardoso, Bernardineli Norberti
Department of Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, São Paulo, Brazil.
Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
J Clin Exp Dent. 2020 Nov 1;12(11):e1058-e1065. doi: 10.4317/jced.57527. eCollection 2020 Nov.
To evaluate the physicochemical properties and cytotoxicity of AH Plus, MTA Fillapex and TotalFill BC Sealer. Volumetric changes were also evaluating using micro-computed tomography (micro-CT).
Radiopacity and flow were evaluated in accordance with the ISO 6876, while setting time was evaluated in accordance with the ASTM- C266-08 specifications. The release of Ca2+ ions and pH were measured with spectrophotometer and pH meter, respectively, after different time intervals (1h, 3h, 24h, 72h, 168h, and 360h). Cytotoxicity was evaluated by MTT reduction assay to check 3T3 cells viability at 24, 48 and 72 hours. Volumetric change was evaluated by micro-CT, by using 30 acrylic teeth, filled with gutta-percha cones and the tested root canal sealer. The samples were evaluated after 168h, 360h and 720h of immersion in distilled water. Data were statistically analyzed by one-way ANOVA and Tukey test or by Kruskal-Wallis and Dunn tests (<0.05).
MTA Fillapex and TotalFill BC Sealer showed lower radiopacity than AH Plus (<0.05). The MTA Fillapex showed the highest flow, while AH Plus showed the lowest flow (<0.05). The initial and final setting time of AH Plus were lower than MTA Fillapex and TotalFill BC Sealer (<0.05). In general, TotalFill BC Sealer presented higher Ca2+ ion release and pH than the other tested sealers. TotalFill BC Sealer also showed overall lower cytotoxicity when compared to the other sealers. Volumetric change of AH Plus and TotalFill BC Sealer was lower than MTA Fillapex (<0.05).
AH Plus, MTA Fillapex and TotalFill BC Sealer showed slight differences in the physicochemical properties and cytotoxicity, but all suitable for an endodontic sealer. However, AH Plus and TotalFill BC Sealer showed low volumetric changes when compared to MTA Fillapex. Calcium silicate, cytotoxicity, physicochemical properties, micro computed tomography.
评估AH Plus、MTA Fillapex和TotalFill BC封闭剂的理化性质及细胞毒性。还使用微型计算机断层扫描（micro-CT）评估体积变化。
按照ISO 6876评估射线不透性和流动性，按照ASTM-C266-08规范评估凝固时间。在不同时间间隔（1小时、3小时、24小时、72小时、168小时和360小时）后，分别用分光光度计和pH计测量Ca2+离子释放量和pH值。通过MTT还原试验评估细胞毒性，以检查3T3细胞在24、48和72小时的活力。使用30颗填充有牙胶尖和受试根管封闭剂的丙烯酸牙，通过micro-CT评估体积变化。将样品在蒸馏水中浸泡168小时、360小时和720小时后进行评估。数据采用单向方差分析和Tukey检验或Kruskal-Wallis和Dunn检验进行统计学分析（<0.05）。
MTA Fillapex和TotalFill BC封闭剂的射线不透性低于AH Plus（<0.05）。MTA Fillapex的流动性最高，而AH Plus的流动性最低（<0.05）。AH Plus的初始和最终凝固时间低于MTA Fillapex和TotalFill BC封闭剂（<0.05）。总体而言，TotalFill BC封闭剂比其他受试封闭剂具有更高的Ca2+离子释放量和pH值。与其他封闭剂相比，TotalFill BC封闭剂的总体细胞毒性也更低。AH Plus和TotalFill BC封闭剂的体积变化低于MTA Fillapex（<0.05）。
AH Plus、MTA Fillapex和TotalFill BC封闭剂在理化性质和细胞毒性方面存在细微差异，但均适用于根管封闭剂。然而，与MTA Fillapex相比，AH Plus和TotalFill BC封闭剂的体积变化较小。硅酸钙、细胞毒性、理化性质、微型计算机断层扫描。
