Hino A, Onitsuka A, Gotoh M, Ozeki Y, Hayashi M, Hirose M, Yoshimi N
First Department of Surgery, Gifu University School of Medicine, Japan.
Nihon Geka Gakkai Zasshi. 1988 Jun;89(6):871-9.
In 25 cases of primary hepatocellular carcinomas, the relationships between clinical, pathological features and DNA histogram patterns were investigated. Nuclear DNA histogram that represents cell cycle and indicates biological malignancy of carcinoma was analyzed by rapid image sensor analytical system scanning enable to measure the density, area and perimeter of nuclei even on thin tissue sections. Histograms of over 65 years old patients showed low ploidy pattern rather than that of less than 65 patients whose AFP values are over 1000 ng/ml showed statistically higher ploidy pattern compared to those of under 10 ng/ml. No significant relations were verified between pathological findings and DNA histogram patterns. But patients who died within 18 months after hepatectomy showed broad and high ploidy histogram patterns compared to patients who lived over 18 months. These results suggested that DNA histogram analysis is useful for evaluate the biological malignancy of primary hepatocellular carcinoma.
对25例原发性肝细胞癌患者的临床、病理特征与DNA直方图模式之间的关系进行了研究。通过快速图像传感器分析系统扫描分析代表细胞周期并指示癌生物学恶性程度的核DNA直方图，该系统即使在薄组织切片上也能测量细胞核的密度、面积和周长。65岁以上患者的直方图显示为低倍体模式，而65岁以下且甲胎蛋白值超过1000 ng/ml的患者与甲胎蛋白值低于10 ng/ml的患者相比，其倍体模式在统计学上更高。病理结果与DNA直方图模式之间未证实有显著关系。但肝切除术后18个月内死亡的患者与存活超过18个月的患者相比，显示出宽而高的倍体直方图模式。这些结果表明，DNA直方图分析有助于评估原发性肝细胞癌的生物学恶性程度。
