Yoon Jaewoo
College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 42601, Republic of Korea.
Arch Microbiol. 2017 Oct;199(8):1133-1139. doi: 10.1007/s00203-017-1384-z. Epub 2017 May 4.
A polyphasic taxonomic study was performed on strain KMU-34, which was isolated from seawater in the Republic of Korea. The bacterial cells were Gram-stain-negative, strictly aerobic, chemoheterotrophic, yellow-pigmented and rod-shaped. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the novel marine strain was affiliated with the family Erythrobacteraceae of the class Alphaproteobacteria and that it showed highest sequence similarity (98.6%) to Erythrobacter atlanticus s21-N3. The DNA-DNA relatedness values between strains KMU-34 and E. atlanticus KCTC 42697 were 8.6 ± 1.2%. The DNA G + C content of strain KMU-34 was determined to be 60.4 mol%. Ubiquinone 10 (Q-10) was the sole respiratory quinone. The predominant cellular fatty acids were C18:1 ω7c (43.8%) and C16:1 ω7c (16.8%). Strain KMU-34 had phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, a sphingoglycolipid, an unidentified phospholipid and two unidentified lipids as polar lipids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel species of the genus Erythrobacter for which the name Erythrobacter alti sp. nov. is proposed. The type strain of E. alti sp. nov. is KMU-34 (= KCCM 90261 = NBRC 111903).
对从韩国海水中分离出的菌株KMU - 34进行了多相分类学研究。该细菌细胞革兰氏染色阴性，严格需氧，化能异养，黄色色素沉着，呈杆状。基于16S rRNA基因序列的系统发育分析表明，该新型海洋菌株隶属于α-变形菌纲红杆菌科，与大西洋红杆菌s21 - N3的序列相似性最高（98.6%）。菌株KMU - 34与大西洋红杆菌KCTC 42697之间的DNA - DNA相关性值为8.6 ± 1.2%。菌株KMU - 34的DNA G + C含量测定为60.4 mol%。泛醌10（Q - 10）是唯一的呼吸醌。主要的细胞脂肪酸为C18:1 ω7c（43.8%）和C16:1 ω7c（16.8%）。菌株KMU - 34含有磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱、一种鞘糖脂、一种未鉴定的磷脂和两种未鉴定的脂质作为极性脂质。基于独特的系统发育位置以及基因型和表型特征的组合，该菌株被认为代表红杆菌属的一个新物种，为此提出新名称高红杆菌（Erythrobacter alti sp. nov.）。高红杆菌新种的模式菌株是KMU - 34（= KCCM 90261 = NBRC 111903）。
