College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu 42601, Republic of Korea.
FEMS Microbiol Lett. 2019 Aug 1;366(15). doi: 10.1093/femsle/fnz182.
A novel alphaproteobacterium, designated KMU-156T, was isolated from seawater collected on the coast of Jeju Island, Republic of Korea, and its phylogenetic position was determined using a polyphasic taxonomic approach. Strain KMU-156T was Gram-stain-negative, strictly aerobic, apricot-colored, rod-shaped, non-motile and chemoorganoheterotrophic. Phylogenetic study based on the 16S rRNA gene sequence revealed that the novel bacterium belongs to the family 'Rhodobacteraceae', of the class Alphaproteobacteria, and that it possessed the greatest sequence similarity (98.2%) with Tateyamaria omphalii MKT107T. DNA-DNA hybridization values between strains KMU-156T, T. omphalii KCTC 12333T and Tateyamaria pelophila DSM 17270T were less than 70%. The major isoprenoid quinone of the novel isolate was ubiquinone-10 (Q-10) and the major (> 10%) cellular fatty acids were C16:0 and C18:1 ω7c. The genomic DNA G + C content of strain KMU-156T was 59.3 mol%. The polar lipid profile of the strain KMU-156T had phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid and two unidentified lipids. From the discriminative taxonomic features, the strain is considered to represent a novel species of the genus Tateyamaria for which the name Tateyamaria armeniaca sp. nov. is proposed. The type strain of T. armeniaca sp. nov. is KMU-156T (= KCCM 90321T = NBRC 113460T).
一株新型的α-变形菌，命名为KMU-156T，从韩国济州岛海岸采集的海水中分离得到，并通过多相分类学方法确定了其系统发育地位。菌株 KMU-156T 革兰氏染色阴性，严格需氧，呈杏黄色，杆状，无动力，化能有机异养。基于 16S rRNA 基因序列的系统发育研究表明，该新型细菌属于 Rhodobacteraceae 科，α-变形菌纲，与 Tateyamaria omphalii MKT107T 的序列相似性最高（98.2%）。菌株 KMU-156T、T. omphalii KCTC 12333T 和 Tateyamaria pelophila DSM 17270T 之间的 DNA-DNA 杂交值小于 70%。新型分离株的主要类异戊二烯醌为泛醌-10（Q-10），主要（>10%）细胞脂肪酸为 C16:0 和 C18:1 ω7c。菌株 KMU-156T 的基因组 DNA G+C 含量为 59.3 mol%。菌株 KMU-156T 的极性脂谱含有磷脂酰甘油、磷脂酰胆碱、一种未鉴定的氨基脂、一种未鉴定的磷脂和两种未鉴定的脂。从区分性分类特征来看，该菌株代表了 Tateyamaria 属的一个新种，命名为 Tateyamaria armeniaca sp. nov.。T. armeniaca sp. nov. 的模式菌株为 KMU-156T（=KCCM 90321T=NBRC 113460T）。
