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新种,从河口水中分离得到。

sp. nov., isolated from isolated from estuary water.

作者信息

Park Sooyeon, Won Sung Min, Yoon Jung-Hoon

机构信息

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.

出版信息

Int J Syst Evol Microbiol. 2019 Sep;69(9):2696-2702. doi: 10.1099/ijsem.0.003510.

Abstract

A Gram-stain-negative, aerobic, non-motile and coccoid-, ovoid- or rod-shaped bacterial strain, designated KEM-5, was isolated from water sampled at an estuary environment on the Yellow Sea, Republic of Korea. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain KEM-5 fell within the clade comprising the type strains of species. Strain KEM-5 exhibited 16S rRNA gene sequence similarities of 97.01-97.66 % to the type strains of , and and of 94.18-96.95 % to the type strains of the other species. The genomicaverage nucleotide identity values of strain KEM-5 with a non-type strain (LAMA 915) of and SW-135 were 76.04 and 74.98 %, respectively. Mean DNA-DNA relatedness values between strain KEM-5 and the type strains of , and were 10-18 %. Strain KEM-5 contained Q-10 as the predominant ubiquinone and C 7, summed feature 3 (Cω7 and/or Cω6), C and Cω6 as the major fatty acids. The major polar lipids of strain KEM-5 were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. The DNA G+C content of strain KEM-5 was 62.4 mol%. Distinguishing phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain KEM-5 is separated from recognized species. On the basis of the data presented here, strain KEM-5 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KEM-5 (=KACC 19865=KCTC 62896=NBRC 113546).

摘要

从韩国黄海河口环境采集的水样中分离出一株革兰氏阴性、需氧、无运动性、呈球状、卵形或杆状的细菌菌株,命名为KEM-5。基于16S rRNA基因序列构建的邻接法系统发育树表明,菌株KEM-5属于包含该属各物种模式菌株的进化分支。菌株KEM-5与该属其他物种的模式菌株的16S rRNA基因序列相似性为97.01 - 97.66%,与其他物种的模式菌株的相似性为94.18 - 96.95%。菌株KEM-5与该属的一株非模式菌株(LAMA 915)以及SW-135的基因组平均核苷酸同一性值分别为76.04%和74.98%。菌株KEM-5与该属模式菌株之间的平均DNA - DNA相关性值为10 - 18%。菌株KEM-5以Q - 10作为主要泛醌,以C7、总特征3(Cω7和/或Cω6)、C和Cω6作为主要脂肪酸。菌株KEM-5的主要极性脂质为磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和鞘糖脂。菌株KEM-5的DNA G + C含量为62.4 mol%。独特的表型特征,以及系统发育和遗传独特性,表明菌株KEM-5与已认可的该属物种不同。基于此处提供的数据,菌株KEM-5被认为代表该属的一个新物种,为此提议命名为新种。模式菌株为KEM-5(=KACC 19865=KCTC 62896=NBRC 113546)。

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