sp. nov., Isolated from the Marine Sponge, , and Reclassification of as comb. nov.

A Gram-stain-negative, facultative anaerobic rods, designated as strain 219JJ12-13, was isolated from a marine sponge, , in Jeju-do, Republic of Korea. The cells displayed catalase and oxidase activity and were non-motile. Strain 219JJ12-13 grew at 10-37°C (optimum, 25-30°C), pH 6.0-8.5 (optimum, pH 7.0-7.5), and in the presence of 0.5-6.0% (w/v) NaCl (optimum, 4.0-5.0%). The polar lipids comprised disphosphatidylglycerol, phosphatidylglycerol, phosphatidylcoline, phosphatidylethanolamine, sphingoglycolipid, two aminophosphoglycolipid, unidentified phospholipid, and two unidentified lipids. The isoprenoid quinone was identified as Q-10, and predominant cellular fatty acids were C 6, summed feature 3 (C 7/C 6), and summed feature 8 (C 7/C18:1 6). The G+C content of the genomic DNA was 63.3%. The 16S rRNA gene and genome sequences-based phylogenetic analyses showed that strain 219JJ12-13 formed a distinct phyletic clade within the genus . Genome relatedness values, including average nucleotide identity and digital DNA-DNA hybridization among strain 219JJ12-13 and closely related type strains, were 74.0-80.2% and 18.2-22.8%, respectively, both markedly below the thresholds for species delineation. Based on polyphasic taxonomic approach, strain 219JJ12-13 represents a novel species of the genus , and the name sp. nov. is proposed. The type strain is 219JJ12-13 (= KACC 23236 = LMG 33060). The reclassification of to the genus as comb. nov. is also proposed (= KCCM 90261 = NBRC 111903).