Kim Soo-Bin, Kim Kyung Hyun, Park Jin-Sook
Department of Biological Sciences and Biotechnology, Hannam University, Daejeon 34430, Republic of Korea.
J Microbiol Biotechnol. 2024 Dec 2;35:e2409010. doi: 10.4014/jmb.2409.09010.
A Gram-stain-negative, facultative anaerobic rods, designated as strain 219JJ12-13, was isolated from a marine sponge, , in Jeju-do, Republic of Korea. The cells displayed catalase and oxidase activity and were non-motile. Strain 219JJ12-13 grew at 10-37°C (optimum, 25-30°C), pH 6.0-8.5 (optimum, pH 7.0-7.5), and in the presence of 0.5-6.0% (w/v) NaCl (optimum, 4.0-5.0%). The polar lipids comprised disphosphatidylglycerol, phosphatidylglycerol, phosphatidylcoline, phosphatidylethanolamine, sphingoglycolipid, two aminophosphoglycolipid, unidentified phospholipid, and two unidentified lipids. The isoprenoid quinone was identified as Q-10, and predominant cellular fatty acids were C 6, summed feature 3 (C 7/C 6), and summed feature 8 (C 7/C18:1 6). The G+C content of the genomic DNA was 63.3%. The 16S rRNA gene and genome sequences-based phylogenetic analyses showed that strain 219JJ12-13 formed a distinct phyletic clade within the genus . Genome relatedness values, including average nucleotide identity and digital DNA-DNA hybridization among strain 219JJ12-13 and closely related type strains, were 74.0-80.2% and 18.2-22.8%, respectively, both markedly below the thresholds for species delineation. Based on polyphasic taxonomic approach, strain 219JJ12-13 represents a novel species of the genus , and the name sp. nov. is proposed. The type strain is 219JJ12-13 (= KACC 23236 = LMG 33060). The reclassification of to the genus as comb. nov. is also proposed (= KCCM 90261 = NBRC 111903).
从韩国济州岛的一种海洋海绵中分离出一株革兰氏阴性兼性厌氧杆菌，命名为219JJ12 - 13菌株。该细胞具有过氧化氢酶和氧化酶活性，无运动性。219JJ12 - 13菌株在10 - 37°C（最适温度为25 - 30°C）、pH 6.0 - 8.5（最适pH为7.0 - 7.5）以及0.5 - 6.0%（w/v）NaCl（最适浓度为4.0 - 5.0%）的条件下生长。极性脂质包括双磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、鞘糖脂、两种氨基磷酸糖脂、未鉴定的磷脂和两种未鉴定的脂质。类异戊二烯醌被鉴定为Q - 10，主要的细胞脂肪酸为C 6、总和特征3（C 7/C 6）和总和特征8（C 7/C18:1 6）。基因组DNA的G + C含量为63.3%。基于16S rRNA基因和基因组序列的系统发育分析表明，219JJ12 - 13菌株在该属内形成了一个独特的进化分支。219JJ12 - 13菌株与密切相关的模式菌株之间的基因组相关性值，包括平均核苷酸同一性和数字DNA - DNA杂交，分别为74.0 - 80.2%和18.2 - 22.8%，均明显低于物种划分的阈值。基于多相分类方法，219JJ12 - 13菌株代表了该属的一个新物种，因此提出新物种名称 sp. nov.。模式菌株为219JJ12 - 13（= KACC 23236 = LMG 33060）。同时还提出将 重新分类到 属，命名为 comb. nov.（= KCCM 90261 = NBRC 111903）。
