Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan.
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan.
Meat Sci. 2017 Sep;131:56-59. doi: 10.1016/j.meatsci.2017.04.009. Epub 2017 Apr 22.
Correct labeling of foods is critical for consumers who wish to avoid a specific meat species for religious or cultural reasons. Therefore, gene-based point-of-care food analysis by real-time Polymerase Chain Reaction (PCR) is expected to contribute to the quality control in the food industry. In this study, we perform rapid identification of meat species by our portable rapid real-time PCR system, following a very simple DNA extraction method. Applying these techniques, we correctly identified beef, pork, chicken, rabbit, horse, and mutton in processed foods in 20min. Our system was sensitive enough to detect the interfusion of about 0.1% chicken egg-derived DNA in a processed food sample. Our rapid real-time PCR system is expected to contribute to the quality control in food industries because it can be applied for the identification of meat species, and future applications can expand its functionality to the detection of genetically modified organisms or mutations.
对于因宗教或文化原因而希望避免特定肉类的消费者来说，正确标注食品至关重要。因此，基于基因的即时聚合酶链反应（PCR）即时检测有望为食品行业的质量控制做出贡献。在这项研究中，我们通过便携式快速实时 PCR 系统，结合非常简单的 DNA 提取方法，快速鉴定肉类物种。应用这些技术，我们在 20 分钟内正确识别了加工食品中的牛肉、猪肉、鸡肉、兔肉、马肉和羊肉。我们的系统足够灵敏，可以检测到加工食品样品中约 0.1%鸡卵源性 DNA 的混合。我们的快速实时 PCR 系统有望为食品行业的质量控制做出贡献，因为它可以用于鉴定肉类物种，未来的应用可以扩展其功能，以检测转基因生物或突变。
